Linked Life Data

12164922

Source:http://linkedlifedata.com/resource/pubmed/id/12164922

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-8-7
pubmed:abstractText
The extracellular matrix is composed of a large number of different modular proteins. Matrilin-2 is a newly described member of the protein superfamily with von Willebrand factor A-like modules. To examine the expression of matrilin-2 in human skin, the distribution of protein and mRNA was studied by immunohistochemistry and in situ hybridization. In addition, immunoblotting and real-time reverse transcription polymerase chain reaction were used to investigate the expression of matrilin-2 in keratinocyte and fibroblast cultures. In vivo, keratinocytes and fibroblasts were both found to express matrilin-2 mRNA and deposit the protein at the basal side of the dermal-epidermal basement membrane. Matrilin-2 molecules synthesized by the two cell types in vitro appeared to be processed differently by cell-associated proteases. Transcription of matrilin-2 mRNA in keratinocytes was enhanced by a diffusible factor produced by fibroblasts, suggesting a regulatory mechanism for the production of extracellular matrix at the dermal-epidermal junction. These findings demonstrate that matrilin-2 is expressed in normal skin by keratinocytes and fibroblasts and may thus contribute to cutaneous homeostasis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-202X
pubmed:author
pubmed:issnType
Print
pubmed:volume
119
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
38-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Expression of matrilin-2 in human skin.
pubmed:affiliation
Department of Dermatology, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 9, 50931 Cologne, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't