| pubmed-article:12163268 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12163268 | lifeskim:mentions | umls-concept:C0021403 | lld:lifeskim |
| pubmed-article:12163268 | lifeskim:mentions | umls-concept:C0079411 | lld:lifeskim |
| pubmed-article:12163268 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:12163268 | pubmed:issue | 25-26 | lld:pubmed |
| pubmed-article:12163268 | pubmed:dateCreated | 2002-8-6 | lld:pubmed |
| pubmed-article:12163268 | pubmed:abstractText | The antigenic variation of influenza A virus hemagglutinin (HA) and neuraminidase (NA) glycoproteins requires frequent changes in vaccine formulation. The classical method of creating influenza virus seed strains for vaccine production is to generate 6 + 2 reassortants that contain six genes from a high-yield virus, such as A/PR/8/34 (H1N1) and the HA and NA genes of the circulating strains. The techniques currently used are time-consuming because of the selection process required to isolate the reassortant virus. We generated the high-yield virus A/PR/8/34 (H1N1) entirely from eight plasmids. Its growth phenotype in embryonated chicken eggs was equivalent to that of the wild-type virus. By using this DNA-based cotransfection technique, we generated 6 + 2 reassortants that had the antigenic determinants of the influenza virus strains A/New Caledonia/20/99 (H1N1), A/Panama/2007/99 (H3N2), A/teal/HK/W312 (H6N1), and A/quail/HK/G1/97 (H9N2). Our findings demonstrate that the eight-plasmid system allows the rapid and reproducible generation of reassortant influenza A viruses for use in the manufacture of vaccines. | lld:pubmed |
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| pubmed-article:12163268 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12163268 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12163268 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12163268 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12163268 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12163268 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12163268 | pubmed:issn | 0264-410X | lld:pubmed |
| pubmed-article:12163268 | pubmed:author | pubmed-author:WebsterRobert... | lld:pubmed |
| pubmed-article:12163268 | pubmed:author | pubmed-author:KraussScottS | lld:pubmed |
| pubmed-article:12163268 | pubmed:author | pubmed-author:HoffmannErich... | lld:pubmed |
| pubmed-article:12163268 | pubmed:author | pubmed-author:PerezDanielD | lld:pubmed |
| pubmed-article:12163268 | pubmed:author | pubmed-author:WebbyRichardR | lld:pubmed |
| pubmed-article:12163268 | pubmed:copyrightInfo | Copyright 2002 Elsevier Science Ltd. | lld:pubmed |
| pubmed-article:12163268 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12163268 | pubmed:day | 19 | lld:pubmed |
| pubmed-article:12163268 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:12163268 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12163268 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12163268 | pubmed:pagination | 3165-70 | lld:pubmed |
| pubmed-article:12163268 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:12163268 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12163268 | pubmed:articleTitle | Eight-plasmid system for rapid generation of influenza virus vaccines. | lld:pubmed |
| pubmed-article:12163268 | pubmed:affiliation | Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 332 North Lauderdale, Memphis, TN 38105-2794, USA. | lld:pubmed |
| pubmed-article:12163268 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12163268 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:12163268 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12163268 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:12163268 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
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