12163268

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0021403, umls-concept:C0079411, umls-concept:C0439831
pubmed:issue	25-26
pubmed:dateCreated	2002-8-6
pubmed:abstractText	The antigenic variation of influenza A virus hemagglutinin (HA) and neuraminidase (NA) glycoproteins requires frequent changes in vaccine formulation. The classical method of creating influenza virus seed strains for vaccine production is to generate 6 + 2 reassortants that contain six genes from a high-yield virus, such as A/PR/8/34 (H1N1) and the HA and NA genes of the circulating strains. The techniques currently used are time-consuming because of the selection process required to isolate the reassortant virus. We generated the high-yield virus A/PR/8/34 (H1N1) entirely from eight plasmids. Its growth phenotype in embryonated chicken eggs was equivalent to that of the wild-type virus. By using this DNA-based cotransfection technique, we generated 6 + 2 reassortants that had the antigenic determinants of the influenza virus strains A/New Caledonia/20/99 (H1N1), A/Panama/2007/99 (H3N2), A/teal/HK/W312 (H6N1), and A/quail/HK/G1/97 (H9N2). Our findings demonstrate that the eight-plasmid system allows the rapid and reproducible generation of reassortant influenza A viruses for use in the manufacture of vaccines.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/AI08831, http://linkedlifedata.com/resource/pubmed/grant/AI29680, http://linkedlifedata.com/resource/pubmed/grant/AI95357, http://linkedlifedata.com/resource/pubmed/grant/CA-21765
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/8406899
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant, http://linkedlifedata.com/resource/pubmed/chemical/Influenza Vaccines
pubmed:status	MEDLINE
pubmed:month	Aug
pubmed:issn	0264-410X
pubmed:author	pubmed-author:HoffmannErichE, pubmed-author:KraussScottS, pubmed-author:PerezDanielD, pubmed-author:WebbyRichardR, pubmed-author:WebsterRobert GRG
pubmed:copyrightInfo	Copyright 2002 Elsevier Science Ltd.
pubmed:issnType	Print
pubmed:day	19
pubmed:volume	20
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	3165-70
pubmed:dateRevised	2007-11-14
pubmed:meshHeading	pubmed-meshheading:12163268-Animals, pubmed-meshheading:12163268-Antigenic Variation, pubmed-meshheading:12163268-Birds, pubmed-meshheading:12163268-Cell Line, pubmed-meshheading:12163268-Chick Embryo, pubmed-meshheading:12163268-China, pubmed-meshheading:12163268-Czechoslovakia, pubmed-meshheading:12163268-DNA, Recombinant, pubmed-meshheading:12163268-Dogs, pubmed-meshheading:12163268-Genes, Viral, pubmed-meshheading:12163268-Genetic Vectors, pubmed-meshheading:12163268-Influenza A virus, pubmed-meshheading:12163268-Influenza Vaccines, pubmed-meshheading:12163268-New Caledonia, pubmed-meshheading:12163268-Panama, pubmed-meshheading:12163268-Phenotype, pubmed-meshheading:12163268-Reassortant Viruses, pubmed-meshheading:12163268-Reproducibility of Results, pubmed-meshheading:12163268-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12163268-Transfection, pubmed-meshheading:12163268-Virus Cultivation
pubmed:year	2002
pubmed:articleTitle	Eight-plasmid system for rapid generation of influenza virus vaccines.
pubmed:affiliation	Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 332 North Lauderdale, Memphis, TN 38105-2794, USA.
pubmed:publicationType	Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Evaluation Studies