Linked Life Data

12163055

Source:http://linkedlifedata.com/resource/pubmed/id/12163055

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2002-8-6
pubmed:abstractText
The hematopoietic supporting abilities are known to be impaired in marrow stromal layers developed from patients with acute myeloid leukemia (AML). In this study, fibroblast growth factor-4 (FGF-4), epidermal growth factor (EGF) or transforming growth factor-beta1 (TGF-beta1) were studied to see whether these growth factors can modify the functional development of leukemic stromal layers. Adherent stromal layers from 13 patients with AML and from six non-leukemic controls were established with 3ng/ml of FGF-4, EGF or TGF-beta1. Established stromal layers were washed three times and irradiated, followed by recharge of allogenic peripheral CD34 positive cells as an indicator of supportive function. Progenitor-outputs into supernatant were evaluated at biweekly interval with colony-forming assay until 6 weeks. The results showed that both leukemic and non-leukemic stromal cells established with FGF-4, but not with EGF, showed significantly higher progenitor cell-outputs compared with control stromal cells. By contrast, stromal cells developed with TGF-beta1 showed significantly lower progenitor cell-outputs compared with control. These differences were significant at later than 4 weeks after the recharge of indicator cells, suggesting that the stromal layer developed with EGF or TGF-beta1 preferentially affected the primitive progenitors rather than committed ones. These results indicate that FGF-4 and TGF-beta1 differentially affect the functional development of leukemic as well as of normal stromal layers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0145-2126
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
933-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12163055-Acute Disease, pubmed-meshheading:12163055-Adult, pubmed-meshheading:12163055-Aged, pubmed-meshheading:12163055-Aged, 80 and over, pubmed-meshheading:12163055-Bone Marrow, pubmed-meshheading:12163055-Cell Communication, pubmed-meshheading:12163055-Cell Count, pubmed-meshheading:12163055-Cell Culture Techniques, pubmed-meshheading:12163055-Cell Division, pubmed-meshheading:12163055-Coculture Techniques, pubmed-meshheading:12163055-Epidermal Growth Factor, pubmed-meshheading:12163055-Female, pubmed-meshheading:12163055-Fibroblast Growth Factor 4, pubmed-meshheading:12163055-Fibroblast Growth Factors, pubmed-meshheading:12163055-Growth Substances, pubmed-meshheading:12163055-Hematopoietic Stem Cells, pubmed-meshheading:12163055-Humans, pubmed-meshheading:12163055-Leukemia, Myeloid, pubmed-meshheading:12163055-Male, pubmed-meshheading:12163055-Middle Aged, pubmed-meshheading:12163055-Proto-Oncogene Proteins, pubmed-meshheading:12163055-Stromal Cells, pubmed-meshheading:12163055-Transforming Growth Factor beta, pubmed-meshheading:12163055-Transforming Growth Factor beta1
pubmed:year
2002
pubmed:articleTitle
Differential effects of fibroblast growth factor-4, epidermal growth factor and transforming growth factor-beta1 on functional development of stromal layers in acute myeloid leukemia.
pubmed:affiliation
Clinical Hematology and Clinical Diagnostics, Graduate School of Medicine, Osaka City University, 1-4-3, Asahimachi, Abeno-ku, Osaka, Japan.
pubmed:publicationType
Journal Article, Comparative Study