Source:http://linkedlifedata.com/resource/pubmed/id/12162677
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2002-8-6
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pubmed:abstractText |
We immunohistochemically examined material from 36 pancreata (adenocarcinomas, 30 lesions; pancreatic intraepithelial neoplasia [PanIN], 65; normal pancreatic ducts, 30) for cyclooxygenase 2 (COX-2) with an automated platform. We analyzed 7 to 10 discrete foci and generated an average percentage of positive cells and average staining intensity for each lesion. These 2 values were then multiplied to create an overall "HistoScore" for each lesion. COX-2 demonstrated considerable heterogeneity of expression between and within cases. The overall average percentage of positive cells in adenocarcinomas was 47.3%; in PanINs, 36.3%; and in normal ducts, 19.2%. COX-2 was expressed in more than 20% of cells in 23 adenocarcinomas (77%), 42 PanINs (65%), and 12 normal ducts (40%). The overall average HistoScore for adenocarcinomas was 6.1; for PanINs, 5.4; and for normal ducts, 3.5. Significant differences in COX-2 expression were demonstrable in adenocarcinomas vs normal ducts, PanINs vs normal ducts, and PanIN 2/3 vs PanIN 1a/1b. In general, the pattern of COX-2 expression increased from normal to PanIN to adenocarcinoma. The up-regulation of COX-2 in a subset of noninvasive precursor lesions makes it a potential target for chemoprevention with selective COX-2 inhibitors.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0002-9173
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
118
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
194-201
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12162677-Adenocarcinoma,
pubmed-meshheading:12162677-Adult,
pubmed-meshheading:12162677-Aged,
pubmed-meshheading:12162677-Aged, 80 and over,
pubmed-meshheading:12162677-Carcinoma in Situ,
pubmed-meshheading:12162677-Cyclooxygenase 2,
pubmed-meshheading:12162677-Female,
pubmed-meshheading:12162677-Humans,
pubmed-meshheading:12162677-Image Processing, Computer-Assisted,
pubmed-meshheading:12162677-Immunohistochemistry,
pubmed-meshheading:12162677-Isoenzymes,
pubmed-meshheading:12162677-Male,
pubmed-meshheading:12162677-Membrane Proteins,
pubmed-meshheading:12162677-Middle Aged,
pubmed-meshheading:12162677-Pancreatic Neoplasms,
pubmed-meshheading:12162677-Precancerous Conditions,
pubmed-meshheading:12162677-Prostaglandin-Endoperoxide Synthases
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pubmed:year |
2002
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pubmed:articleTitle |
Cyclooxygenase 2 expression in pancreatic adenocarcinoma and pancreatic intraepithelial neoplasia: an immunohistochemical analysis with automated cellular imaging.
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pubmed:affiliation |
Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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