Source:http://linkedlifedata.com/resource/pubmed/id/12153712
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2002-8-2
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pubmed:abstractText |
Assembly of the human signal recognition particle (SRP) requires SRP19 protein to bind to helices 6 and 8 of SRP RNA. In the present study, structure of a 29-mer RNA composing the SRP19 binding site in helix 6 was determined by NMR spectroscopy. The two A:C mismatches were continuously stacked to each other and formed wobble type A:C base pairs. The GGAG tetraloop in helix 6 was found to adopt a similar conformation to that of GNRA tetraloop, suggesting that these tetraloops are included in an extensive new motif GNRR. Compared with the crystal structure of helix 6 in complex with SRP19 determined previously, the GGAG tetraloop in the complex was found to adopt a similar conformation to the free form, although the loop structure becomes more open upon SRP19 binding. Thus, SRP19 is thought to recognize the overall fold of the GGAG loop.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-924X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
132
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-82
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:12153712-Binding Sites,
pubmed-meshheading:12153712-Humans,
pubmed-meshheading:12153712-Models, Molecular,
pubmed-meshheading:12153712-Molecular Structure,
pubmed-meshheading:12153712-Nuclear Magnetic Resonance, Biomolecular,
pubmed-meshheading:12153712-Nucleic Acid Conformation,
pubmed-meshheading:12153712-Protein Binding,
pubmed-meshheading:12153712-Protein Structure, Tertiary,
pubmed-meshheading:12153712-RNA, Small Cytoplasmic,
pubmed-meshheading:12153712-Signal Recognition Particle
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pubmed:year |
2002
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pubmed:articleTitle |
Solution structure of a SRP19 binding domain in human SRP RNA.
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pubmed:affiliation |
Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology, Narashino, Chiba 275-0016, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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