Source:http://linkedlifedata.com/resource/pubmed/id/12153174
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2002-8-2
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| pubmed:abstractText |
The purpose of the present study was to characterize the human Helios gene products expressed in leukemia cells. A 3.5 kb human Helios cDNA clone was isolated from a human T-cell cDNA library derived from the human T-acute lymphoblastic leukemia (ALL) cell line JURKAT. This cDNA clone had a unique open reading frame (ORF) encoding a novel 304 amino acid (AA) peptide, which was designated as Helios 3. The sequence of the 289 AA C-terminal portion of Helios 3 downstream of V-16 is identical to the corresponding sequence found in Helios 1 and 2 and contains two zinc fingers. By contrast, the 15 AA N-terminal portion of Helios 3 is unique and does not contain the N-terminal zinc finger motifs that are conserved in Helios 1 and 2 as well as other previously identified members of the Ikaros family. Southern blot analysis of genomic DNA fragments of the human Helios gene locus showed that Helios 3 is encoded by the same genomic locus as Helios 1 and 2. The expression of Helios 3 mRNA was not restricted to T-lineage ALL cells or another immunophenotypically distinct subset of leukemias. Helios 3 mRNA was expressed in freshly obtained primary leukemic cells from six of 15 children with newly diagnosed ALL. Helios 3 exhibited a unique protein interaction profile via its N-terminal portion, which may have biological significance in pathogenesis of human leukemias.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/IKZF2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Ikaros Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
1042-8194
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
43
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
841-9
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:12153174-Adolescent,
pubmed-meshheading:12153174-Amino Acid Sequence,
pubmed-meshheading:12153174-Base Sequence,
pubmed-meshheading:12153174-Blotting, Northern,
pubmed-meshheading:12153174-Child,
pubmed-meshheading:12153174-Child, Preschool,
pubmed-meshheading:12153174-Cloning, Molecular,
pubmed-meshheading:12153174-DNA-Binding Proteins,
pubmed-meshheading:12153174-Female,
pubmed-meshheading:12153174-Gene Expression,
pubmed-meshheading:12153174-Humans,
pubmed-meshheading:12153174-Ikaros Transcription Factor,
pubmed-meshheading:12153174-Leukemia,
pubmed-meshheading:12153174-Male,
pubmed-meshheading:12153174-Molecular Sequence Data,
pubmed-meshheading:12153174-Protein Isoforms,
pubmed-meshheading:12153174-RNA, Messenger,
pubmed-meshheading:12153174-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12153174-Transcription Factors,
pubmed-meshheading:12153174-Tumor Cells, Cultured
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| pubmed:year |
2002
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| pubmed:articleTitle |
Expression of a unique helios isoform in human leukemia cells.
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| pubmed:affiliation |
Department of Molecular Biology, Parker Hughes Cancer Center, Parker Hughes Institute, St Paul, MN 55113, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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