Linked Life Data

12153026

Source:http://linkedlifedata.com/resource/pubmed/id/12153026

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2002-8-2
pubmed:abstractText
Application of wastewater to soil may result in the contamination of groundwater and soil with pathogenic microorganisms and other biological and chemical agents. This study was performed to determine the antiviral microbial activity of soil saturated with secondary effluent. Low concentrations (0.05mg/ml) of protease pronase resulted in the inactivation of more than 90% of seeded Cox-A9 virus, whereas Poliovirus type 1, Hepatitis A virus (HAV) and MS2 bacteriophages were found to be insensitive to the enzyme activity. Exposure of Cox A9 virus to P. aeruginosa extracellular enzymes resulted in 99% inactivation of the seeded virus. Hepatitis A virus was found to be as sensitive as the Cox A9 virus, whereas Poliovirus 1 and MS2 were found to be insensitive to P. aeruginosa extracellular enzymatic activity. Furthermore, the time required for 99% reduction (T99) of Cox A9 and MS-2 Bacteriophage, at 15 degrees C, in soil saturated with secondary effluent was found to be 7 and 21 days, respectively. Faster inactivation was observed for MS2 and Cox A9 in soil saturated with secondary effluent incubated at 30 degrees C, T99 of 2 and 0.3 days, respectively. Although the concentration of the total bacterial count in the soil samples increased from 10(3) cfu/g to 10(5) cfu/g after 20 days of incubation at 30 degrees C, the proteolytic activity was below the detection level. The results of this study indicate that the virucidal effect of microbial activity is virus type dependent. Furthermore microbial activity in the soil material can be enhanced by the application of secondary effluent at higher temperature. The results also showed that MS2 bacteriophage can be used to predict viral contamination of soil and groundwater.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0043-1354
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2589-95
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Contribution of microbial activity to virus reduction in saturated soil.
pubmed:affiliation
Water Quality Research Laboratory, National Public Health Laboratories, Ministry of Health, Tel-Aviv, Israel. abid.nasser@phlta.health.gov.il
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't