Linked Life Data

12151238

Source:http://linkedlifedata.com/resource/pubmed/id/12151238

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-8-1
pubmed:abstractText
Pichia etchellsii CBS2011 (synonym Debaryomyces etchellsii) is a non-killer yeast harbouring two cryptic linear cytoplasmic DNA-elements, pPE1A (6.7 kb) and pPE1B (12.8 kb). Cloning and complete sequencing of pPE1A revealed a 6749-bp element with a remarkably high A+T content of 77.6%. The termini of pPE1A were found to consist of inversely orientated identical nucleotide repetitions of 178bp, to which proteins are linked at the 5'-ends. It is only the second small, non-autonomous cytoplasmic yeast linear plasmid for which the complete nucleotide sequence is known. Five open reading frames (ORFs) were identified preceded by upstream conserved sequence motifs (UCS) characteristic for cytoplasmic promoters and perfectly matching the UCS consensus (ATNTGA). As none of the putative genes encodes a DNA-polymerase, pPE1A is the first yeast linear plasmid known that does not possess its own element-specific replication machinery. No function could be attributed to ORF1, 3, 4, and 5; the predicted ORF2 gene product is similar to chitin-binding proteins and chitinases, highest homologies were found to the precursor of the alpha- and beta-subunits of the secreted Kluyveromyces lactis zymocin. Consistently, the Orf2p could be isolated from the culture fluid by chitin-Sepharose affinity chromatography and characterized by immuno-probing with an antibody specific for the K. lactis killer toxin alpha-subunit. Production of the protein was found to be plasmid-dependent. The sequence of pPE1A has been submitted to the EMBL data library, Accession No. AJ409097.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0147-619X
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
224-33
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12151238-Amino Acid Sequence, pubmed-meshheading:12151238-Base Sequence, pubmed-meshheading:12151238-Blotting, Western, pubmed-meshheading:12151238-Chitin, pubmed-meshheading:12151238-Chromatography, Affinity, pubmed-meshheading:12151238-Cloning, Molecular, pubmed-meshheading:12151238-Fungal Proteins, pubmed-meshheading:12151238-Genome, pubmed-meshheading:12151238-Killer Factors, Yeast, pubmed-meshheading:12151238-Kluyveromyces, pubmed-meshheading:12151238-Molecular Sequence Data, pubmed-meshheading:12151238-Mycotoxins, pubmed-meshheading:12151238-Open Reading Frames, pubmed-meshheading:12151238-Pichia, pubmed-meshheading:12151238-Plasmids, pubmed-meshheading:12151238-Protein Binding, pubmed-meshheading:12151238-Protein Subunits, pubmed-meshheading:12151238-Sequence Analysis, DNA, pubmed-meshheading:12151238-Sequence Homology, Amino Acid
pubmed:year
2002
pubmed:articleTitle
Genome organization of the linear Pichia etchellsii plasmid pPE1A: evidence for expression of an extracellular chitin-binding protein homologous to the alpha-subunit of the Kluyveromyces lactis killer toxin.
pubmed:affiliation
Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, Corrensstr. 3, D-48149, Münster, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't