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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-8-1
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pubmed:databankReference |
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pubmed:abstractText |
AMY-1 has been identified by us as a c-Myc-binding protein and was found to stimulate c-Myc transcription activity. AMY-1 was also found to be associated with AKAP84/149 in the mitochondria in somatic cells and sperm, suggesting that it plays a role in spermatogenesis. To access the molecular function of AMY-1, a two-hybrid screening of cDNAs encoding AMY-1-binding proteins was carried out with AMY-1 as a bait using a human testis cDNA library, and a clone encoding a novel protein, AMAP-1, was obtained. The amap-1 gene was mapped at human chromosome 17q21. AMY-1 was found to bind to and be colocalized with AMAP-1 in human 293T and HeLa cells. AMAP-1 was found to be specifically expressed in the testis and expressed post-meiotically in the testis, as was AMY-1. These results suggest that both AMAP-1 and AMY-1 play roles in spermatogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
1577
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
126-32
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12151104-Amino Acid Sequence,
pubmed-meshheading:12151104-Animals,
pubmed-meshheading:12151104-Base Sequence,
pubmed-meshheading:12151104-Carrier Proteins,
pubmed-meshheading:12151104-Chromosomes, Human, Pair 17,
pubmed-meshheading:12151104-DNA-Binding Proteins,
pubmed-meshheading:12151104-Gene Expression Regulation,
pubmed-meshheading:12151104-HeLa Cells,
pubmed-meshheading:12151104-Humans,
pubmed-meshheading:12151104-Kinetics,
pubmed-meshheading:12151104-Male,
pubmed-meshheading:12151104-Mice,
pubmed-meshheading:12151104-Molecular Sequence Data,
pubmed-meshheading:12151104-RNA, Messenger,
pubmed-meshheading:12151104-Salivary alpha-Amylases,
pubmed-meshheading:12151104-Spermatogenesis,
pubmed-meshheading:12151104-Testis,
pubmed-meshheading:12151104-Tissue Distribution,
pubmed-meshheading:12151104-Transcription, Genetic,
pubmed-meshheading:12151104-Transcription Factors
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pubmed:year |
2002
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pubmed:articleTitle |
AMAP-1, a novel testis-specific AMY-1-binding protein, is differentially expressed during the course of spermatogenesis.
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pubmed:affiliation |
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan.
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pubmed:publicationType |
Journal Article
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