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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
34
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pubmed:dateCreated |
2002-7-31
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pubmed:abstractText |
Protein kinase CK2 (formerly casein kinase II) is frequently upregulated in human cancers, and transgenic expression of CK2alpha in lymphocytes is oncogenic. Lymphomagenesis is dramatically accelerated by co-expression of a c-myc transgene, suggestive of a synergistic interaction between the kinase and the transcription factor. Since c-myc can be phosphorylated by CK2, we hypothesized that the synergy between CK2 and c-myc might be due to a functional interaction of the two molecules. Pharmacologic inhibition of CK2 activity in cell lines established from CK2alpha transgenic T cell lymphomas reduces their proliferation and concomitantly with this, the steady state levels of c-myc protein decline. This is caused by accelerated c-myc protein turnover, which occurs in a proteasome-dependent manner. Transfection of cells with sense or anti-sense CK2 constructs modulates c-myc protein levels in concert with the alteration in CK2 activity, validating the findings obtained using the kinase inhibitors. Thus, CK2 is a critical regulator of c-myc protein stability and of the proliferation of these T cell lymphomas.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5280-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12149649-Animals,
pubmed-meshheading:12149649-Apigenin,
pubmed-meshheading:12149649-Blotting, Western,
pubmed-meshheading:12149649-Casein Kinase II,
pubmed-meshheading:12149649-Cell Division,
pubmed-meshheading:12149649-Cells, Cultured,
pubmed-meshheading:12149649-Emodin,
pubmed-meshheading:12149649-Flavonoids,
pubmed-meshheading:12149649-Glutathione Transferase,
pubmed-meshheading:12149649-Lymphoma,
pubmed-meshheading:12149649-Mice,
pubmed-meshheading:12149649-Mice, Transgenic,
pubmed-meshheading:12149649-Peptide Hydrolases,
pubmed-meshheading:12149649-Phosphorylation,
pubmed-meshheading:12149649-Plasmids,
pubmed-meshheading:12149649-Protein-Serine-Threonine Kinases,
pubmed-meshheading:12149649-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:12149649-Recombinant Fusion Proteins,
pubmed-meshheading:12149649-T-Lymphocytes
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pubmed:year |
2002
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pubmed:articleTitle |
Functional interaction of protein kinase CK2 and c-Myc in lymphomagenesis.
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pubmed:affiliation |
Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts, MA 02118, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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