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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-7-31
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pubmed:abstractText |
The phenotype induced by the GATA-1(low) (neodeltaHS) mutation is here further characterized by analyzing the hemopoietic system during the aging (up to 20 months) of a GATA-1(low) colony (135 mutants and 40 normal littermates). Mutants expressed normal hematocrit values (Hct = 45.9 +/- 4.0) until 12 months but became anemic from 15 months on (Hct = 30.9 +/- 3.9; P <.05). Anemia was associated with several markers of myelofibrosis such as the presence of tear-drop poikilocytes and progenitor cells in the blood, collagen fibers in the marrow and in the spleen, and hemopoietic foci in the liver. Semiquantitative reverse transcription-polymerase chain reaction showed that growth factor genes implicated in the development of myelofibrosis (such as osteocalcin, transforming growth factor-beta1, platelet-derived growth factor, and vascular endothelial growth factor) were all expressed in the marrow from the mutants at higher levels than in corresponding normal tissues. The GATA-1(low) mutants experienced a slow progression of the disease because the final exitus was not observed until at least 15 months with a probability of survival more favorable than that of W/Wv mice concurrently kept in the animal facility (P <.001, by Kaplan-Meier analysis). In conclusion, impaired GATA-1 expression may contribute to the development of myelofibrosis, and the GATA-1(low) mutants may represent a suitable animal model for the human disease that may shed light on its pathogenesis.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Erythroid-Specific DNA-Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/GATA1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Gata1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Osteocalcin,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
100
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1123-32
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12149188-Anemia,
pubmed-meshheading:12149188-Animals,
pubmed-meshheading:12149188-Bone Marrow,
pubmed-meshheading:12149188-Collagen,
pubmed-meshheading:12149188-DNA-Binding Proteins,
pubmed-meshheading:12149188-Endothelial Growth Factors,
pubmed-meshheading:12149188-Erythrocytes,
pubmed-meshheading:12149188-Erythroid Precursor Cells,
pubmed-meshheading:12149188-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:12149188-Female,
pubmed-meshheading:12149188-Fibroblast Growth Factor 1,
pubmed-meshheading:12149188-GATA1 Transcription Factor,
pubmed-meshheading:12149188-Gene Expression,
pubmed-meshheading:12149188-Hematocrit,
pubmed-meshheading:12149188-Hematopoiesis, Extramedullary,
pubmed-meshheading:12149188-Liver,
pubmed-meshheading:12149188-Lymphokines,
pubmed-meshheading:12149188-Male,
pubmed-meshheading:12149188-Mice,
pubmed-meshheading:12149188-Mice, Inbred C57BL,
pubmed-meshheading:12149188-Mice, Mutant Strains,
pubmed-meshheading:12149188-Mutation,
pubmed-meshheading:12149188-Osteocalcin,
pubmed-meshheading:12149188-Platelet-Derived Growth Factor,
pubmed-meshheading:12149188-Primary Myelofibrosis,
pubmed-meshheading:12149188-Spleen,
pubmed-meshheading:12149188-Transcription Factors,
pubmed-meshheading:12149188-Transforming Growth Factor beta,
pubmed-meshheading:12149188-Vascular Endothelial Growth Factor A,
pubmed-meshheading:12149188-Vascular Endothelial Growth Factors
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pubmed:year |
2002
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pubmed:articleTitle |
Development of myelofibrosis in mice genetically impaired for GATA-1 expression (GATA-1(low) mice).
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pubmed:affiliation |
Department of Ematologia, University of Florence, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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