Linked Life Data

12147494

Source:http://linkedlifedata.com/resource/pubmed/id/12147494

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2002-7-30
pubmed:abstractText
Moraxella osloensis is a gram-negative bacterium associated with Phasmarhabditis hermaphrodita, a slug-parasitic nematode that has prospects for biological control of mollusk pests, especially the grey garden slug, Deroceras reticulatum. This bacterium-feeding nematode acts as a vector that transports M. osloensis into the shell cavity of the slug, and the bacterium is the killing agent in the nematode-bacterium complex. We discovered that M. osloensis produces an endotoxin(s), which is tolerant to heat and protease treatments and kills the slug after injection into the shell cavity. Washed or broken cells treated with penicillin and streptomycin from 3-day M. osloensis cultures were more pathogenic than similar cells from 2-day M. osloensis cultures. However, heat and protease treatments and 2 days of storage at 22 degrees C increased the endotoxin activity of the young broken cells but not the endotoxin activity of the young washed cells treated with the antibiotics. This suggests that there may be a proteinaceous substance(s) that is structurally associated with the endotoxin(s) and masks its toxicity in the young bacterial cells. Moreover, 2 days of storage of the young washed bacterial cells at 22 degrees C enhanced their endotoxin activity if they were not treated with the antibiotics. Furthermore, purified lipopolysaccharide (LPS) from the 3-day M. osloensis cultures was toxic to slugs, with an estimated 50% lethal dose of 48 microg per slug, thus demonstrating that the LPS of M. osloensis is an endotoxin that is active against D. reticulatum. This appears to be the first report of a biological toxin that is active against mollusks.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12147494-10596805, http://linkedlifedata.com/resource/pubmed/commentcorrection/12147494-11679319, http://linkedlifedata.com/resource/pubmed/commentcorrection/12147494-11780820, http://linkedlifedata.com/resource/pubmed/commentcorrection/12147494-11781271, http://linkedlifedata.com/resource/pubmed/commentcorrection/12147494-1908833, http://linkedlifedata.com/resource/pubmed/commentcorrection/12147494-7107844, http://linkedlifedata.com/resource/pubmed/commentcorrection/12147494-7558327, http://linkedlifedata.com/resource/pubmed/commentcorrection/12147494-8075270, http://linkedlifedata.com/resource/pubmed/commentcorrection/12147494-8243038, http://linkedlifedata.com/resource/pubmed/commentcorrection/12147494-9152030, http://linkedlifedata.com/resource/pubmed/commentcorrection/12147494-9173208
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3943-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Endotoxin activity of Moraxella osloensis against the grey garden slug, Deroceras reticulatum.
pubmed:affiliation
Department of Entomology, Ohio State University, Ohio Agricultural Research and Development Center, Wooster, Ohio 44691, USA. tan.100@osu.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't