Linked Life Data

12146942

Source:http://linkedlifedata.com/resource/pubmed/id/12146942

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
31
pubmed:dateCreated
2002-7-30
pubmed:abstractText
SmtB is required for Synechococcus to effect a response to toxic concentrations of Zn(II) and other heavy metals. Direct binding of inducing metal ions to SmtB transcriptionally derepresses the expression of SmtA, a prokaryotic class II metallothionein. Homodimeric SmtB binds one Zn(II) or Co(II) per monomer in a cysteine thiolate-containing site in a tetrahedral coordination geometry [VanZile, M. L., et al. (2000) Biochemistry 39, 11818-11829]. In this report, characterization of a set of cysteine substitution mutants of SmtB reveals that SmtB homodimer binds Zn(II) or Co(II) in one of two mutually exclusive metal binding sites, termed alpha3N and alpha5, with very high equilibrium affinities. Both sites are characterized by similar affinities for Co(II) (K(Co) approximately equal to 2-5 x 10(9) M(-1)), while the Zn(II) affinities are at least 20-fold different (K(Zn)(alpha)(3N) > or = 10(13) M(-1); K(Zn)(alpha)(5) approximately equal to 5 x 10(11) M(-1)). Co(II) bound exclusively at the alpha5 sites is capable of rapid equilibration between the alpha3N and alpha5 sites upon reduction of the mixed disulfides in S-methylated SmtB. These results suggest that the alpha3N or alpha5 metal sites might play distinct roles in this Zn(II)-sensing protein, systematically investigated in the following paper [VanZile, M. L., Chen, X., and Giedroc, D. P. (2002) Biochemistry 41, 9776-9786]. Since both the alpha3N and alpha5 sites are present in many members of the SmtB/ArsR family of metal sensor proteins, the presence of these two metal binding sites may explain some of the functional diversity in metal responses across this family of proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9765-75
pubmed:dateRevised
2009-7-17
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Structural characterization of distinct alpha3N and alpha5 metal sites in the cyanobacterial zinc sensor SmtB.
pubmed:affiliation
Department of Biochemistry and Biophysics, Center for Advanced Biomolecular Research, Texas A&M University, College Station, TX 77843-2128, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't