Source:http://linkedlifedata.com/resource/pubmed/id/12144771
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2002-7-29
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pubmed:abstractText |
Flavescence dorée is a devastating disease of grapevine widespread in several countries in EU such as France, Italy and Spain. Genetic variability among 17 Italian and 3 French FD strains was investigated by RFLP analyses based on a fragment of the ribosomal protein operon and on the non-ribosomal DNA fragment FD9. RFLP analysis of the PCR amplified ribosomal protein fragment, coding for the 3' end of rpl22 and the entire rps3 genes, differentiated 4 rp-subgroups among the FD strains and 4 subgroups among the reference strains belonging to elm yellows group (16SrV). Sequencing and phylogenetic analysis of the same ribosomal protein DNA fragment validated the delineation of 4 distinct FD strain types derived by RFLP analyses. The results supported the differentiation based on analysis of the non-ribosomal DNA fragment FD9. The phylogenetic analysis further revealed relationships and a probable evolutionary trend among the FD strains and the other representatives of elm yellows group. All the FD strains together with the reference strains ALY, RuS and JWB formed a cluster very well distinct from the EY/ULW cluster. Moreover, ALY was shown to be more closely related to three FD strain types: the Lombardia/Piemonte, the French FD70, and the French FD88/Italian FD-D strain clusters.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0890-8508
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
197-208
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12144771-DNA, Bacterial,
pubmed-meshheading:12144771-DNA, Ribosomal,
pubmed-meshheading:12144771-France,
pubmed-meshheading:12144771-Genetic Variation,
pubmed-meshheading:12144771-Italy,
pubmed-meshheading:12144771-Molecular Sequence Data,
pubmed-meshheading:12144771-Mycoplasma,
pubmed-meshheading:12144771-Operon,
pubmed-meshheading:12144771-Phylogeny,
pubmed-meshheading:12144771-Plant Diseases,
pubmed-meshheading:12144771-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:12144771-Sequence Alignment,
pubmed-meshheading:12144771-Vitis
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pubmed:year |
2002
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pubmed:articleTitle |
Genetic variability among flavescence dorée phytoplasmas from different origins in Italy and France.
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pubmed:affiliation |
DiSTA, Patologia Vegetale, University of Bologna, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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