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pubmed-article:12143302pubmed:issue7lld:pubmed
pubmed-article:12143302pubmed:dateCreated2002-7-29lld:pubmed
pubmed-article:12143302pubmed:abstractTextHS2 and HS3 are important elements of human beta-globin locus control region (LCR). To study the effect of HS2, HS3, and HS2-HS3 on human beta-globin gene expression, a series of expression cassettes were constructed, in which the reporter gene encoding the enhanced green fluorescent protein (EGFP) was driven by the beta-globin promotor and under the control of HS2, HS3, and HS2-HS3. These constructs were transfected transiently into MEL and K562 cell lines mediated with liposome and their expression was measured with FACS as well as semi-quantitative RT-PCR method. The results showed that 3.2-kb HS2 could significantly enhance the activity of beta-globin promotor in MEL and K562 cells, while 3-kb HS3 could do only in MEL cells. There was no synergistic function in transient expression in the cassettes with the combination of HS2 and HS3 in MEL and K562 cells.lld:pubmed
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pubmed-article:12143302pubmed:statusMEDLINElld:pubmed
pubmed-article:12143302pubmed:monthJullld:pubmed
pubmed-article:12143302pubmed:issn0379-4172lld:pubmed
pubmed-article:12143302pubmed:authorpubmed-author:JiaChun-PingC...lld:pubmed
pubmed-article:12143302pubmed:authorpubmed-author:HuangShu-Zhen...lld:pubmed
pubmed-article:12143302pubmed:authorpubmed-author:ZengYi-TaoYTlld:pubmed
pubmed-article:12143302pubmed:issnTypePrintlld:pubmed
pubmed-article:12143302pubmed:volume29lld:pubmed
pubmed-article:12143302pubmed:ownerNLMlld:pubmed
pubmed-article:12143302pubmed:authorsCompleteYlld:pubmed
pubmed-article:12143302pubmed:pagination565-70lld:pubmed
pubmed-article:12143302pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:12143302pubmed:year2002lld:pubmed
pubmed-article:12143302pubmed:articleTitle[A study of the effect of locus control region elements HS2 and HS3 on beta-globin gene expression].lld:pubmed
pubmed-article:12143302pubmed:affiliationShanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai 200040, China.lld:pubmed
pubmed-article:12143302pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12143302pubmed:publicationTypeEnglish Abstractlld:pubmed
pubmed-article:12143302pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed