Linked Life Data

12143302

Source:http://linkedlifedata.com/resource/pubmed/id/12143302

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2002-7-29
pubmed:abstractText
HS2 and HS3 are important elements of human beta-globin locus control region (LCR). To study the effect of HS2, HS3, and HS2-HS3 on human beta-globin gene expression, a series of expression cassettes were constructed, in which the reporter gene encoding the enhanced green fluorescent protein (EGFP) was driven by the beta-globin promotor and under the control of HS2, HS3, and HS2-HS3. These constructs were transfected transiently into MEL and K562 cell lines mediated with liposome and their expression was measured with FACS as well as semi-quantitative RT-PCR method. The results showed that 3.2-kb HS2 could significantly enhance the activity of beta-globin promotor in MEL and K562 cells, while 3-kb HS3 could do only in MEL cells. There was no synergistic function in transient expression in the cassettes with the combination of HS2 and HS3 in MEL and K562 cells.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0379-4172
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
565-70
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
[A study of the effect of locus control region elements HS2 and HS3 on beta-globin gene expression].
pubmed:affiliation
Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai 200040, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't