Linked Life Data

12140734

Source:http://linkedlifedata.com/resource/pubmed/id/12140734

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2002-7-25
pubmed:abstractText
Keratinocyte growth factor (KGF) stimulates epithelial cell differentiation and proliferation, which are of major importance for wound healing. Local protein administration, however, has been shown to be ineffective due to enzymes and proteases in the wound fluid. We hypothesized that delivering KGF as a non-viral liposomal cDNA gene complex is a new approach that would effectively enhance dermal and epidermal regeneration. Twenty-two rats were given an acute wound and divided into two groups to receive weekly subcutaneous injections of liposomes plus the LacZ gene (0.2 microg, vehicle), or liposomes plus the KGF cDNA (2.2 microg) and LacZ cDNA (0.2 microg). Transfection was confirmed by histochemical assays for beta-galactosidase. Planimetry, histological and immunohistochemical techniques were used to determine protein expression, dermal and epidermal regeneration. Transfection and subsequent KGF expression was found in diving cells in the granulation tissue. Epidermal regeneration was improved by 170% in rats receiving the KGF cDNA constructs by exhibiting the most rapid area and linear wound re-epithelialization, P < 0.0001. KGF improved epidermal cell net balance by increasing skin cell proliferation and decreasing skin cell apoptosis, P < 0.0001. Dermal regeneration was further improved in KGF cDNA treated animals by an increased collagen deposition and morphology, P < 0.0001. KGF cDNA increased neo-vascularization and concomitant VEGF concentrations when compared with vehicle, P < 0.01. KGF cDNA did not only stimulate epithelial cells, but also mesenchymal cells through increases in IGF-I concentration, P < 0.005. Liposomes containing the KGF cDNA gene constructs were effective in improving epidermal and dermal regeneration. KGF gene transfer to acute wounds may represent a new therapeutic strategy to enhance wound healing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0969-7128
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1065-74
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12140734-Animals, pubmed-meshheading:12140734-Apoptosis, pubmed-meshheading:12140734-Burns, pubmed-meshheading:12140734-Cell Division, pubmed-meshheading:12140734-Collagen, pubmed-meshheading:12140734-DNA, Complementary, pubmed-meshheading:12140734-Epidermis, pubmed-meshheading:12140734-Fibroblast Growth Factor 7, pubmed-meshheading:12140734-Fibroblast Growth Factors, pubmed-meshheading:12140734-Gene Therapy, pubmed-meshheading:12140734-Gene Transfer Techniques, pubmed-meshheading:12140734-Insulin-Like Growth Factor I, pubmed-meshheading:12140734-Liposomes, pubmed-meshheading:12140734-Male, pubmed-meshheading:12140734-Rats, pubmed-meshheading:12140734-Rats, Sprague-Dawley, pubmed-meshheading:12140734-Regeneration, pubmed-meshheading:12140734-Skin, pubmed-meshheading:12140734-Skin Physiological Phenomena, pubmed-meshheading:12140734-Transfection, pubmed-meshheading:12140734-Wound Healing
pubmed:year
2002
pubmed:articleTitle
Non-viral liposomal keratinocyte growth factor (KGF) cDNA gene transfer improves dermal and epidermal regeneration through stimulation of epithelial and mesenchymal factors.
pubmed:affiliation
Klinik und Poliklinik für Chirurgie, University of Regensburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't