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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
25
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pubmed:dateCreated |
2002-7-24
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pubmed:abstractText |
A xylanase with a molecular weight of 15 kDa, which is lower than those of previously reported xylanases, was isolated from the roots of the medicinal herb Panax notoginseng. The xylanase exhibits a temperature optimum of 50 degrees C and a pH optimum between 5 and 6. It inhibits HIV-1 reverse transcriptase with an IC(50) of 10 microM, but does not affect translation in a cell-free rabbit reticulocyte system when tested up to 70 microM. The enzyme is adsorbed on CM-cellulose, Affi-gel blue gel and Mono S. Previously xylanases have been isolated from seeds and not from roots, and have not been demonstrated to inhibit HIV-1 reverse transcriptase.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0024-3205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3049-58
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12138018-Chromatography,
pubmed-meshheading:12138018-HIV Reverse Transcriptase,
pubmed-meshheading:12138018-Humans,
pubmed-meshheading:12138018-Panax,
pubmed-meshheading:12138018-Plant Extracts,
pubmed-meshheading:12138018-Plant Roots,
pubmed-meshheading:12138018-Plants, Medicinal,
pubmed-meshheading:12138018-Protein Biosynthesis,
pubmed-meshheading:12138018-Reverse Transcriptase Inhibitors,
pubmed-meshheading:12138018-Xylan Endo-1,3-beta-Xylosidase,
pubmed-meshheading:12138018-Xylosidases
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pubmed:year |
2002
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pubmed:articleTitle |
A xylanase from roots of sanchi ginseng (Panax notoginseng) with inhibitory effects on human immunodeficiency virus-1 reverse transcriptase.
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pubmed:affiliation |
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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