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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
41
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pubmed:dateCreated |
2002-10-7
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pubmed:databankReference |
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pubmed:abstractText |
We have isolated the novel gene SMOC-1 that encodes a secreted modular protein containing an EF-hand calcium-binding domain homologous to that in BM-40. It further consists of two thyroglobulin-like domains, a follistatin-like domain and a novel domain. Recombinant expression in human cells showed that SMOC-1 is a glycoprotein with a calcium-dependent conformation. Results from Northern blots, reverse transcriptase-PCR, and immunoblots revealed a widespread expression in many tissues. Immunofluorescence studies with an antiserum directed against recombinant human SMOC-1 demonstrated a basement membrane localization of the protein and additionally its presence in other extracellular matrices. Immunogold electron microscopy confirmed the localization of SMOC-1 within basement membranes in kidney and skeletal muscle as well as its expression in the zona pellucida surrounding the oocyte.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
37977-86
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12130637-Amino Acid Sequence,
pubmed-meshheading:12130637-Animals,
pubmed-meshheading:12130637-Base Sequence,
pubmed-meshheading:12130637-Basement Membrane,
pubmed-meshheading:12130637-Calcium-Binding Proteins,
pubmed-meshheading:12130637-Circular Dichroism,
pubmed-meshheading:12130637-EF Hand Motifs,
pubmed-meshheading:12130637-Female,
pubmed-meshheading:12130637-Glycoproteins,
pubmed-meshheading:12130637-Humans,
pubmed-meshheading:12130637-Kidney,
pubmed-meshheading:12130637-Laminin,
pubmed-meshheading:12130637-Mice,
pubmed-meshheading:12130637-Molecular Sequence Data,
pubmed-meshheading:12130637-Muscle, Skeletal,
pubmed-meshheading:12130637-Osteonectin,
pubmed-meshheading:12130637-Ovary,
pubmed-meshheading:12130637-Recombinant Proteins,
pubmed-meshheading:12130637-Tissue Distribution,
pubmed-meshheading:12130637-Zona Pellucida
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pubmed:year |
2002
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pubmed:articleTitle |
Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes.
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pubmed:affiliation |
Institute for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann Strasse 52, D-50931 Cologne, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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