12130578

pubmed:Citation

8

While the orphan nuclear receptor steroidogenic factor-1 (SF-1) has been shown to function as an induction factor to define adrenocortical cell lineage, it remains unclear whether SF-1 plays an additional role as a growth promoting agent in the adult adrenal cortex. The proliferative potential of the adrenal cortex in adult SF-1(+/-) mice was examined using the model of compensatory adrenal growth following unilateral adrenalectomy (uADX). While the right adrenal gland of wild-type (wt) mice grew significantly after uADX, the adrenal of SF-1(+/-) mice exhibited a blunted, statistically nonsignificant weight increase. Accordingly, a profound increase in the proliferation marker proliferating cell nuclear antigen could be detected only in wt mice after uADX but not in the SF-1(+/-) mice. The proposed key regulator in adrenal compensatory growth, the recently cloned adrenal secretory serine protease was up-regulated in the remaining adrenal of wt mice, whereas this increase was blunted in SF-1(+/-) mice. While no differences in preadipocyte factor-1, the presumed marker of primitive adrenocortical cells, were detectable in the adrenals of wt and SF-1(+/-) mice, an increase in the ACTH receptor as well as agouti-related protein was observed only in wt animals but not in the SF-1(+/-) mice following uADX. Taken together, these results reflect a primary inability of adrenal cortical cells of SF-1(+/-) mice to undergo compensatory adrenal growth in response to uADX.

eng

AIM

MEDLINE

0013-7227

Print

NLM

3122-35

pubmed-meshheading:12130578-Adrenal Glands, pubmed-meshheading:12130578-Adrenalectomy, pubmed-meshheading:12130578-Adrenocorticotropic Hormone, pubmed-meshheading:12130578-Agouti-Related Protein, pubmed-meshheading:12130578-Animals, pubmed-meshheading:12130578-Corticosterone, pubmed-meshheading:12130578-DNA-Binding Proteins, pubmed-meshheading:12130578-Fushi Tarazu Transcription Factors, pubmed-meshheading:12130578-Homeodomain Proteins, pubmed-meshheading:12130578-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:12130578-Male, pubmed-meshheading:12130578-Membrane Proteins, pubmed-meshheading:12130578-Mice, pubmed-meshheading:12130578-Mice, Inbred DBA, pubmed-meshheading:12130578-Proliferating Cell Nuclear Antigen, pubmed-meshheading:12130578-Protein Biosynthesis, pubmed-meshheading:12130578-Proteins, pubmed-meshheading:12130578-RNA, Messenger, pubmed-meshheading:12130578-Receptors, Corticotropin, pubmed-meshheading:12130578-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:12130578-Repressor Proteins, pubmed-meshheading:12130578-Serine Endopeptidases, pubmed-meshheading:12130578-Steroidogenic Factor 1, pubmed-meshheading:12130578-Transcription Factors, pubmed-meshheading:12130578-Ventromedial Hypothalamic Nucleus

Steroidogenic factor-1 is essential for compensatory adrenal growth following unilateral adrenalectomy.

Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't