Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2002-7-19
pubmed:databankReference
pubmed:abstractText
In order to isolate genes regulating sex differentiation of embryonic gonads, we have attempted to obtain genes specifically expressed in male embryonic gonads by means of subtractive hybridization screening, and we have cloned a novel mouse gene, Tdl. It potentially encodes a protein showing sequence homology to anti-microbial protein, beta-defensins. Tdl reveals structural features such as the six cysteine residues with invariant spacing, which are found in beta-defensins, but the overall amino acid similarity of Tdl to other members of the beta-defensin family is low. In addition, the Tdl gene shows genomic organization similar to that of other beta-defensin genes. We have found that Tdl is specifically expressed in Sertoli cell-lineage in seminiferous cords in embryonic testes, but not in embryonic ovaries after 12.5dpc when the sexual differentiation of gonads is initiated. Tdl is specifically expressed in gonads among adult tissues, and its expression persisted in Sertoli cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0925-4773
pubmed:author
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
217-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12128228-Amino Acid Sequence, pubmed-meshheading:12128228-Animals, pubmed-meshheading:12128228-Base Sequence, pubmed-meshheading:12128228-DNA, Complementary, pubmed-meshheading:12128228-Female, pubmed-meshheading:12128228-Gene Expression Regulation, Developmental, pubmed-meshheading:12128228-In Situ Hybridization, pubmed-meshheading:12128228-Male, pubmed-meshheading:12128228-Mice, pubmed-meshheading:12128228-Molecular Sequence Data, pubmed-meshheading:12128228-Ovary, pubmed-meshheading:12128228-RNA, Messenger, pubmed-meshheading:12128228-Sequence Homology, Amino Acid, pubmed-meshheading:12128228-Sertoli Cells, pubmed-meshheading:12128228-Sex Differentiation, pubmed-meshheading:12128228-Testis, pubmed-meshheading:12128228-Tissue Distribution, pubmed-meshheading:12128228-beta-Defensins
pubmed:year
2002
pubmed:articleTitle
Testis-specific expression of a novel mouse defensin-like gene, Tdl.
pubmed:affiliation
Graduate School of Medicine, Osaka University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't