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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23-24
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pubmed:dateCreated |
2002-7-19
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pubmed:abstractText |
Synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs trigger an immune response characterized by the activation of B cells, NK cells and monocytes/macrophages. Based on evidence that the immunogenicity of DNA vaccines can be augmented by the addition of CpG motifs, 5-20 additional CpG motifs were cloned into a pUC-derived plasmid. Treating bone-marrow derived dendritic cells (BM-DCs) with CpG-enriched plasmids in vitro boosted their expressions of MHC class II molecules, the CD40 and CD86 activation markers. Co-administering the CpG-enriched plasmids with a DNA vaccine encoding the envelope glycoprotein of HIV to BALB/c mice significantly increased HIV-specific cell mediated and humoral immunity. A significant boost was observed when the CpG plasmid was administered either 2 or 4 days after DNA vaccination. Plasmids containing 20 CpG copies were the most effective immune enhancers both in vitro and in vivo. These results suggest that plasmids containing multiple CpG motifs may improve the immunogenicity of DNA vaccines.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AIDS Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/CPG-oligonucleotide,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp120,
http://linkedlifedata.com/resource/pubmed/chemical/HIV envelope protein gp120 (305-321),
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-410X
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pubmed:author |
pubmed-author:HamajimaKenjiK,
pubmed-author:HoshinoYukaY,
pubmed-author:JounaiNaoN,
pubmed-author:KlinmanDennisD,
pubmed-author:KojimaYoshitsuguY,
pubmed-author:NakazawaMasatoshiM,
pubmed-author:OikawaTomohiroT,
pubmed-author:OkudaKenjiK,
pubmed-author:OokiTakaakiT,
pubmed-author:OzakiTomomiT,
pubmed-author:ShinodaKaoriK,
pubmed-author:XinKe-QinKQ
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2857-65
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12126895-AIDS Vaccines,
pubmed-meshheading:12126895-Adjuvants, Immunologic,
pubmed-meshheading:12126895-Amino Acid Sequence,
pubmed-meshheading:12126895-Animals,
pubmed-meshheading:12126895-Antigen Presentation,
pubmed-meshheading:12126895-Antigens, CD,
pubmed-meshheading:12126895-Antigens, CD40,
pubmed-meshheading:12126895-Antigens, CD86,
pubmed-meshheading:12126895-Base Sequence,
pubmed-meshheading:12126895-CpG Islands,
pubmed-meshheading:12126895-Dendritic Cells,
pubmed-meshheading:12126895-HIV Envelope Protein gp120,
pubmed-meshheading:12126895-Histocompatibility Antigens Class II,
pubmed-meshheading:12126895-Hypersensitivity, Delayed,
pubmed-meshheading:12126895-Interferon-gamma,
pubmed-meshheading:12126895-Male,
pubmed-meshheading:12126895-Membrane Glycoproteins,
pubmed-meshheading:12126895-Mice,
pubmed-meshheading:12126895-Mice, Inbred BALB C,
pubmed-meshheading:12126895-Molecular Sequence Data,
pubmed-meshheading:12126895-Oligodeoxyribonucleotides,
pubmed-meshheading:12126895-Peptide Fragments,
pubmed-meshheading:12126895-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:12126895-Vaccines, DNA
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pubmed:year |
2002
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pubmed:articleTitle |
Adjuvant effect of multi-CpG motifs on an HIV-1 DNA vaccine.
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pubmed:affiliation |
Department of Bacteriology, University School of Medicine, Yokohama City, Yokohama 236-0004, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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