Linked Life Data

12121436

Source:http://linkedlifedata.com/resource/pubmed/id/12121436

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-7-17
pubmed:abstractText
Intermolecular spreading of humoral autoimmunity to different components of the Ro (SS-A) and La (SS-B) ribonucleoprotein (RNP) complex has been reported following immunization with a single component of the complex. Although the immune response to the immunizing antigen is polyclonal and diversified, little is known about the specificity of the recruited autoimmune responses to the endogenous Ro and La antigens which drive B-cell spreading. To determine the specificity of intermolecular spreading to La, we examined sera from 52 kDa Ro (Ro52)- and 60 kDa Ro (Ro60)-immunized C3H/HeJ mice for reactivity with recombinant fragments spanning endogenous mouse (m)La by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Sera from mice primed and boosted with recombinant Ro52 and Ro60 showed reactivity restricted to the COOH-terminal fragment of mLa (aa361-415). The recruited anti-La response was species-specific, cross-reacting weakly with the corresponding region on the human La molecule, and was abrogated by the preabsorption of the Ro-immune sera with mLa 361-415. Analogous experiments using recombinant mRo60 fragments spanning the mRo60 molecule revealed a similar pattern of oligoclonality in the specificity of anti-Ro60 autoimmunity following active immunization with La and Ro52. These results suggest that intermolecular-intrastructural T-B help is limiting in this model, and reveal unsuspected immunodominance of selected Ro-La epitopes in the spreading of the autoantibody response to these structures. The focusing of the recruited autoantibody response to these COOH-terminal regions of the Ro and La polypeptides may also reflect the surface accessibility of these regions in La-Ro RNP.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Antinuclear, http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens, http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, B-Lymphocyte, http://linkedlifedata.com/resource/pubmed/chemical/Immunodominant Epitopes, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Cytoplasmic, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins, http://linkedlifedata.com/resource/pubmed/chemical/SS-A antibodies, http://linkedlifedata.com/resource/pubmed/chemical/SS-A antigen, http://linkedlifedata.com/resource/pubmed/chemical/SS-B antibodies, http://linkedlifedata.com/resource/pubmed/chemical/SS-B antigen, http://linkedlifedata.com/resource/pubmed/chemical/TROVE2 protein, human
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0300-9475
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
168-73
pubmed:dateRevised
2007-5-11
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Restricted specificity of intermolecular spreading to endogenous La (SS-B) and 60 kDa Ro (SS-A) in experimental autoimmunity.
pubmed:affiliation
Department of Immunology, Allergy and Arthritis, Flinders Medical Centre and University, Adelaide, Australia. imtg@flinders.edu.au
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't