Linked Life Data

12121221

Source:http://linkedlifedata.com/resource/pubmed/id/12121221

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-7-17
pubmed:abstractText
Monocyte extravasation initiates reorganization of the cytoskeleton (CSK) and adhesion-dependent cytokine gene transcription. The actin CSK is thought to be crucial for compartmentalization and translation of mRNA, many of which contain AU-rich (ARE) instability motifs in the 3' untranslated region. We investigated regulation of adhesion-induced IL-1 beta expression by the monocyte CSK. In serum-free adherent monocytes, the induced IL-1 beta mRNA was stable and did not coextract with actin filaments. In contrast, in cells adherent in autologous serum, IL-1 beta transcripts were unstable, coextracted with actin filaments and were associated with only transient activation of the mitogen-activated protein kinases (MAPK). Under both conditions of adherence, the ARE-binding protein AUF1/hnRNP D was readily extracted in the cytosolic fraction. Electro-injection with AUF1/hnRNP D modified the actin CSK and, surprisingly, stabilized IL-1 beta transcripts. These data suggest that the control of mRNA degradation is linked with changes in the CSK. Mitogen-activated protein kinase activation or alterations in the availability of mRNA degradation factors may mediate these effects.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0818-9641
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
328-39
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:12121221-Actin Cytoskeleton, pubmed-meshheading:12121221-Cell Adhesion, pubmed-meshheading:12121221-Cells, Cultured, pubmed-meshheading:12121221-Culture Media, pubmed-meshheading:12121221-Cytoskeletal Proteins, pubmed-meshheading:12121221-Heterogeneous-Nuclear Ribonucleoprotein D, pubmed-meshheading:12121221-Humans, pubmed-meshheading:12121221-Interleukin-1, pubmed-meshheading:12121221-Intermediate Filaments, pubmed-meshheading:12121221-Kinetics, pubmed-meshheading:12121221-Mitogen-Activated Protein Kinases, pubmed-meshheading:12121221-Monocytes, pubmed-meshheading:12121221-RNA, Messenger, pubmed-meshheading:12121221-RNA Stability, pubmed-meshheading:12121221-RNA-Binding Proteins, pubmed-meshheading:12121221-Signal Transduction, pubmed-meshheading:12121221-Transcription, Genetic
pubmed:year
2002
pubmed:articleTitle
IL-1 beta transcript stability in monocytes is linked to cytoskeletal reorganization and the availability of mRNA degradation factors.
pubmed:affiliation
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't