Linked Life Data

12119554

Source:http://linkedlifedata.com/resource/pubmed/id/12119554

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-7-16
pubmed:abstractText
Prostate cancer is one of the most common neoplasms in the USA and Europe. We used differential display PCR (DD-PCR) to identify androgen-regulated genes in prostate cancer. The RNA of LNCaP cells treated with dihydrotestosterone (DHT) was analyzed for differentially expressed genes. Using DD-PCR, we identified a down-regulated cDNA fragment by DHT in LNCaP cells. This fragment was cloned and expressions of this fragment in prostate cancer cell lines were analyzed by RT-PCR. Sequence analysis revealed that a cDNA fragment is identical to protein tyrosine phosphatase LAR related gene, liprin-alpha2. liprin-alpha2 was downregulated by dihydrotestosterone (DHT) in LNCaP cells in a time- and androgen concentration-dependent manner. Downregulation by DHT was not inhibited by the protein synthesis inhibitor cycloheximide. This liprin-alpha2 gene was not expressed in androgen independent prostate cancer cell lines PC-3 and DU-145 at the mRNA level. And also, we first revealed here that liprin-alpha2 mRNA is expressed in LNCaP cells as well as human prostate cancer tissues and normal prostate tissues. These data suggest that liprin-alpha2 might play a role in androgen responsive human prostate cancer cell line as well as human prostate cells, and the loss of this gene expression might be associated with the androgen independent characteristics of prostate cancer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1107-3756
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
173-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12119554-Adaptor Proteins, Signal Transducing, pubmed-meshheading:12119554-Adenocarcinoma, pubmed-meshheading:12119554-Androgens, pubmed-meshheading:12119554-Cycloheximide, pubmed-meshheading:12119554-DNA, Complementary, pubmed-meshheading:12119554-Dihydrotestosterone, pubmed-meshheading:12119554-Down-Regulation, pubmed-meshheading:12119554-Gene Expression Regulation, Neoplastic, pubmed-meshheading:12119554-Humans, pubmed-meshheading:12119554-Male, pubmed-meshheading:12119554-Membrane Proteins, pubmed-meshheading:12119554-Neoplasm Proteins, pubmed-meshheading:12119554-Neoplasms, Hormone-Dependent, pubmed-meshheading:12119554-Prostate, pubmed-meshheading:12119554-Prostatic Neoplasms, pubmed-meshheading:12119554-Protein Synthesis Inhibitors, pubmed-meshheading:12119554-RNA, Messenger, pubmed-meshheading:12119554-RNA, Neoplasm, pubmed-meshheading:12119554-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12119554-Tumor Cells, Cultured
pubmed:year
2002
pubmed:articleTitle
Liprin-alpha2 gene, protein tyrosine phosphatase LAR interacting protein related gene, is downregulated by androgens in the human prostate cancer cell line LNCaP.
pubmed:affiliation
Department of Urology, Yokohama City University, Yokohama, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't