Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-7-15
pubmed:abstractText
To investigate the relative importance of inhaled particulates and soluble components in the response to inhaled hay dust suspension (HDS), heaves (previously termed chronic obstructive pulmonary disease; n = 7) and control (n = 6) horses were given inhalation challenges with whole and fractionated HDS. Fractionation was achieved by centrifugation to yield supernatant (SUP) and particulate debris. The particulates were then washed repeatedly in saline to produce a washed particulate (WP) fraction which comprised mainly fungal spores, and a wash fraction (WF) which comprised saline and soluble components washed from the surface of the particulates. Inhalation of HDS and SUP induced a significant airway neutrophilia in both groups, with the magnitude of the response being significantly greater in heaves horses. SUP induced significantly less airway neutrophilia than HDS in both groups, despite the endotoxin and protease content of HDS and SUP being comparable. WP and WF induced only a slight airway neutrophilia in heaves horses. However, a combined challenge with SUP and WP induced a neutrophilic response approaching the magnitude of that following HDS challenge, indicating that dust particulates contribute to the pulmonary recruitment of neutrophils in heaves. Consequently, inhalation challenge with HDS, which contains both particulates and soluble dust components, may be a more useful tool for the diagnosis and investigation of heaves than aqueous dust extracts, which contain only soluble components.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0425-1644
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
343-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12117105-Animals, pubmed-meshheading:12117105-Case-Control Studies, pubmed-meshheading:12117105-Chemical Fractionation, pubmed-meshheading:12117105-Diagnosis, Differential, pubmed-meshheading:12117105-Dose-Response Relationship, Immunologic, pubmed-meshheading:12117105-Dust, pubmed-meshheading:12117105-Female, pubmed-meshheading:12117105-Horse Diseases, pubmed-meshheading:12117105-Horses, pubmed-meshheading:12117105-Inhalation Exposure, pubmed-meshheading:12117105-Leukocyte Count, pubmed-meshheading:12117105-Male, pubmed-meshheading:12117105-Nebulizers and Vaporizers, pubmed-meshheading:12117105-Neutrophils, pubmed-meshheading:12117105-Particle Size, pubmed-meshheading:12117105-Pulmonary Disease, Chronic Obstructive, pubmed-meshheading:12117105-Respiratory Function Tests, pubmed-meshheading:12117105-Solubility, pubmed-meshheading:12117105-Spores, Fungal, pubmed-meshheading:12117105-Suspensions
pubmed:year
2002
pubmed:articleTitle
Evaluation of nebulised hay dust suspensions (HDS) for the diagnosis and investigation of heaves. 3: Effect of fractionation of HDS.
pubmed:affiliation
Wellcome Trust Centre for Research in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, Roslin, Midlothian, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies