Source:http://linkedlifedata.com/resource/pubmed/id/12115952
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2002-7-12
|
| pubmed:abstractText |
The fate of macrophages infiltrating damaged rat skeletal muscle fibers after intramuscular injection of the anesthetic bupivacaine hydrochloride (BPVC) and the possible roles of monocyte chemoattractant protein-1 (MCP-1) were investigated. The number of macrophages reached a maximum level at 2 days after the injection and then gradually decreased. The number of apoptotic cells detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay was elevated at 2-4 days and decreased thereafter. In serial sections, TUNEL-positive cells were also immunopositive for RM-4, an antibody specific for identification of macrophages. Electron microscopy further confirmed that it was the macrophages themselves that were undergoing apoptosis. As assessed by reverse transcriptase-polymerase chain reaction (RT-PCR), high levels of MCP-1 mRNA in BPVC-treated muscles were observed and positively correlated with maximum macrophage infiltration. However, the levels of MCP-1 mRNA returned to normal low values coincident with decrease of inflammation and healing of damaged muscle fiber. Local apoptosis of macrophages, under the control of MCP-1, may be involved in healing of BPVC-treated muscles.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anesthetics, Local,
http://linkedlifedata.com/resource/pubmed/chemical/Bupivacaine,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0148-639X
|
| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2002 Wiley Periodicals, Inc.
|
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
79-86
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:12115952-Anesthetics, Local,
pubmed-meshheading:12115952-Animals,
pubmed-meshheading:12115952-Apoptosis,
pubmed-meshheading:12115952-Bupivacaine,
pubmed-meshheading:12115952-Chemokine CCL2,
pubmed-meshheading:12115952-Immunohistochemistry,
pubmed-meshheading:12115952-In Situ Nick-End Labeling,
pubmed-meshheading:12115952-Inflammation,
pubmed-meshheading:12115952-Injections, Intramuscular,
pubmed-meshheading:12115952-Lipopolysaccharides,
pubmed-meshheading:12115952-Lung,
pubmed-meshheading:12115952-Macrophages,
pubmed-meshheading:12115952-Male,
pubmed-meshheading:12115952-Microscopy, Electron,
pubmed-meshheading:12115952-Muscle, Skeletal,
pubmed-meshheading:12115952-Muscle Fibers, Skeletal,
pubmed-meshheading:12115952-RNA, Messenger,
pubmed-meshheading:12115952-Rats,
pubmed-meshheading:12115952-Rats, Wistar,
pubmed-meshheading:12115952-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12115952-Spleen
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Macrophage apoptosis in rat skeletal muscle treated with bupivacaine hydrochloride: possible role of MCP-1.
|
| pubmed:affiliation |
Department of Orthopedic Surgery, Osaka Medical College, Takatsuki, Osaka, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|