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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2002-7-12
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pubmed:abstractText |
We previously demonstrated that immunotherapy with dendritic cells (DC) prevented diabetes development in prediabetic NOD mice and that this effect was optimal when using a stimulatory DC population generated from bone marrow cells cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. In this study, we have investigated the mechanism by which GM-CSF- and IL-4-cultured DC prevent diabetes in prediabetic NOD mice. Histological analysis of pancreatic tissue from DC-treated mice revealed a reduction in the severity of insulitis compared to controls. Analysisof the T cell response in DC-treated mice suggested a general shift towards a Th2-dominated response, as determined by cytokine production following either concanavalin A or anti-TCR stimulation. Furthermore, sorted CD45RB(lo) CD25+ CD4+ T cells from the spleen of DC-treated mice produced high amounts of Th2 cytokines following anti-TCR stimulation, suggesting that these cells are responsible for the apparent Th2 shift. We conclude that DC therapy may have corrected the immunoregulatory defect in the NOD mouse, thus restoring a balance between pathogenic Th1 cells and protective Th2 cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2021-30
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12115623-Animals,
pubmed-meshheading:12115623-Antigens, CD45,
pubmed-meshheading:12115623-Biological Markers,
pubmed-meshheading:12115623-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12115623-Cytokines,
pubmed-meshheading:12115623-Dendritic Cells,
pubmed-meshheading:12115623-Diabetes Mellitus, Type 1,
pubmed-meshheading:12115623-Female,
pubmed-meshheading:12115623-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:12115623-Immunotherapy, Adoptive,
pubmed-meshheading:12115623-Interleukin-4,
pubmed-meshheading:12115623-Mice,
pubmed-meshheading:12115623-Mice, Inbred NOD,
pubmed-meshheading:12115623-Pancreas,
pubmed-meshheading:12115623-Protein Tyrosine Phosphatase, Non-Receptor Type 1,
pubmed-meshheading:12115623-Receptors, Interleukin-2,
pubmed-meshheading:12115623-Th2 Cells
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pubmed:year |
2002
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pubmed:articleTitle |
Regulatory Th2 response induced following adoptive transfer of dendritic cells in prediabetic NOD mice.
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pubmed:affiliation |
Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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