Linked Life Data

12113987

Source:http://linkedlifedata.com/resource/pubmed/id/12113987

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-7-12
pubmed:abstractText
The improvement in the sensitive determination method of estradiol using HPLC with electrochemical detection is described. The improvement was due to the optimization of the potential applied to the electrode of the analytical cell and employment of a guard cell. The detection conditions were optimized from the electrochemical properties of estradiol in acidic and alkaline eluents. The employment of the guard cell drastically decreased the background noise without any reduction in the response of estradiol, and contributed to improvement in the sensitivity. The optimized method combined with pretreatment by liquid-liquid extraction was applied to the determination of estradiol in rat plasma. The detection limit of 8 pg for the standard solution and 24 pg for the plasma sample, which was about 6-8-fold more sensitive compared to the previous reports, was attained.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1570-0232
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Elsevier Science BV.
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
775
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-13
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Sensitive determination method of estradiol in plasma using high-performance liquid chromatography with electrochemical detection.
pubmed:affiliation
Kashima Pharmaceutical Development Laboratories, Mitsubishi Pharma Corporation, 14-Sunayama, Hasaki-machi, Kashima-gun, Ibaraki 314-0255, Japan. yamada.hiroyuki@mm.m-pharma.co.jp
pubmed:publicationType
Journal Article