Linked Life Data

12111550

Source:http://linkedlifedata.com/resource/pubmed/id/12111550

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-7-11
pubmed:abstractText
During cultivation in the presence of N-acetylglucosamine or chitin, Streptomyces olivaceoviridis mycelium efficiently takes up [(14)C]-labelled N-acetylglucosamine. Uptake of the labelled compound can be completely inhibited by unlabelled N-acetylglucosamine and partially by chitobiose. After extraction of the membrane with Triton X-100, two forms of a protein that binds to N-acetylglucosamine and N, N'-diacetylchitobiose (chitobiose) were purified to homogeneity by two consecutive rounds of anionic exchange chromatography. The protein was named NgcE. Using surface plasmon resonance, its binding parameters were determined. It showed highest affinity for N-acetylglucosamine (K(D)=8.28 x 10(-9) M) and for chitobiose (K(D)=2.87 x 10(-8) M). Varying equilibrium dissociation constants in the micromolecular range were ascertained for chitotetraose (K(D)=4.5 x 10(-6) M), chitopentaose (K(D)=1.03 x 10(-6) M) and chitohexaose (K(D)=3.02 x 10(-6) M); the lowest value was measured for chitotriose (K(D)=19.4 x 10(-6) M). After having determined the sequences of several internal peptides from the binding protein by Edman degradation, the corresponding ngcE gene, which encodes a predicted lipid-anchored protein, was identified by reverse genetics. Using a genomic phage library of S. olivaceoviridis genes encoding two other membrane proteins (named NgcF and NgcG) were identified adjacent to ngcE. Each of these is predicted to have six membrane-spanning helices and a consensus motif for integral membrane proteins characteristic of ABC transporters. In addition, the gene for a predicted regulator protein (NgcR) was detected. The ngcEFG operon lacks a gene for an ATP-hydrolysing protein. NgcE is a new member of the CUT-1 family of ABC transporters for carbohydrates. Comparative studies of the wild-type and a mutant strain carrying an insertion within the ngc operon clearly demonstrate that the Ngc system mediates the uptake of N-acetylglucosamine and chitobiose in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1617-4615
pubmed:author
pubmed:issnType
Print
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
429-39
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12111550-ATP-Binding Cassette Transporters, pubmed-meshheading:12111550-Acetylglucosamine, pubmed-meshheading:12111550-Amino Acid Sequence, pubmed-meshheading:12111550-Bacterial Proteins, pubmed-meshheading:12111550-Base Sequence, pubmed-meshheading:12111550-Biological Transport, Active, pubmed-meshheading:12111550-Cloning, Molecular, pubmed-meshheading:12111550-DNA, Bacterial, pubmed-meshheading:12111550-Disaccharides, pubmed-meshheading:12111550-Genes, Bacterial, pubmed-meshheading:12111550-Kinetics, pubmed-meshheading:12111550-Molecular Sequence Data, pubmed-meshheading:12111550-Mutation, pubmed-meshheading:12111550-Operon, pubmed-meshheading:12111550-Peptide Library, pubmed-meshheading:12111550-Protein Binding, pubmed-meshheading:12111550-Sequence Homology, Amino Acid, pubmed-meshheading:12111550-Streptomyces, pubmed-meshheading:12111550-Surface Plasmon Resonance
pubmed:year
2002
pubmed:articleTitle
The novel Streptomyces olivaceoviridis ABC transporter Ngc mediates uptake of N-acetylglucosamine and N,N'-diacetylchitobiose.
pubmed:affiliation
Fachbereich Biologie/Chemie, Barbarastr. 11, D-49069 Osnabrück, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't