Linked Life Data

12110704

Source:http://linkedlifedata.com/resource/pubmed/id/12110704

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-7-11
pubmed:abstractText
We previously showed that hypoxia (1% O(2)) favors the self-renewal of murine and human normal hematopoietic stem cells. This study represents the first attempt to characterize the effects of hypoxia on the maintenance of chronic myeloid leukemia (CML) progenitors. CD34(+) cells isolated from apheresis products of CML patients were incubated in hypoxia (1% O(2)) and normoxia (20% O(2)). After 8 days of culture, their proliferation, capacity for colony-forming-cell (CFC) generation in secondary cultures (pre-CFC), and phenotype (CD34 and platelet-activating factor receptor [PAF-R]) were compared with those of normal cells, and tyrosine phosphorylation in CML cells was measured. Hypoxia inhibits the proliferation of CD34(+) cells and preserves the pre-CFC capacity and cell-surface CD34 expression of CML cells better than normoxia. The PAF-R expression, which was absent on freshly isolated cells, was detected at the cell surface in both populations after 8 days of culture, but with a lower percentage of positive cells in CML cell cultures. Incubation in hypoxia suppressed the PAF-R expression of normal cells and increased it in CML cells, resulting in a similar expression in the two populations. These effects could be linked to inhibition by hypoxia of the tyrosine hyperphosphorylation of cellular proteins, a major hallmark of CML cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1066-5099
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
347-54
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12110704-Antigens, CD34, pubmed-meshheading:12110704-Cell Differentiation, pubmed-meshheading:12110704-Cell Division, pubmed-meshheading:12110704-Cell Hypoxia, pubmed-meshheading:12110704-Cell Membrane, pubmed-meshheading:12110704-Cell Survival, pubmed-meshheading:12110704-Down-Regulation, pubmed-meshheading:12110704-Gene Expression, pubmed-meshheading:12110704-Humans, pubmed-meshheading:12110704-Leukemia, Myelogenous, Chronic, BCR-ABL Positive, pubmed-meshheading:12110704-Neoplastic Stem Cells, pubmed-meshheading:12110704-Phosphorylation, pubmed-meshheading:12110704-Platelet Membrane Glycoproteins, pubmed-meshheading:12110704-Receptors, Cell Surface, pubmed-meshheading:12110704-Receptors, G-Protein-Coupled, pubmed-meshheading:12110704-Tumor Cells, Cultured, pubmed-meshheading:12110704-Tyrosine
pubmed:year
2002
pubmed:articleTitle
Hypoxia modifies proliferation and differentiation of CD34(+) CML cells.
pubmed:affiliation
Laboratoire de Greffe de Moelle, Université Bordeaux 2, Bordeaux, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't