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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-7-10
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pubmed:abstractText |
Cardiovascular disease is a serious complication in diabetic patients. To elucidate the precise mechanisms of atherosclerosis in diabetic patients, the effects of high glucose concentration (25 mM) on apoptosis regulation and bcl-2 family protein expression in human coronary artery smooth muscle cells (CASMC) were examined. Treatment with a high level of glucose (25 mM) caused a significant decrease in apoptosis in CASMC compared with the same cells treated with a physiologically normal glucose concentration (5.5 mM) (23.9 +/- 2.4% vs. 16.5 +/- 1.8%; P < 0.01). With respect to apoptosis regulation, treatment of CASMC with high glucose concentration markedly increased mRNA expressions of bcl-xL and bfl-1/A1 compared with cells treated with normal glucose. High glucose induced phosphorylation of phosphatidylinositol 3-kinase (PI 3-K) and extracellular signal-regulated kinase (ERK)1/2 along with bcl-xL and bfl-1/A1 upregulation. These results suggest that high glucose suppresses apoptosis via upregulation of bcl-xL and bfl-1/A1 levels through PI 3-K and ERK1/2 pathways in CASMC. High glucose-induced increase in the expression of antiapoptotic proteins may be important in the development of atherosclerosis in diabetic patients.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0363-6143
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
283
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
C422-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12107051-Apoptosis,
pubmed-meshheading:12107051-Arteries,
pubmed-meshheading:12107051-Cells, Cultured,
pubmed-meshheading:12107051-Coronary Vessels,
pubmed-meshheading:12107051-Dose-Response Relationship, Drug,
pubmed-meshheading:12107051-Enzyme Activation,
pubmed-meshheading:12107051-Glucose,
pubmed-meshheading:12107051-Humans,
pubmed-meshheading:12107051-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:12107051-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:12107051-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12107051-Muscle, Smooth, Vascular,
pubmed-meshheading:12107051-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:12107051-Phosphorylation,
pubmed-meshheading:12107051-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:12107051-Up-Regulation,
pubmed-meshheading:12107051-bcl-X Protein
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pubmed:year |
2002
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pubmed:articleTitle |
High glucose inhibits apoptosis in human coronary artery smooth muscle cells by increasing bcl-xL and bfl-1/A1.
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pubmed:affiliation |
Third Department of Internal Medicine, Gifu University School of Medicine, Gifu 500-8705, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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