Linked Life Data

12106590

Source:http://linkedlifedata.com/resource/pubmed/id/12106590

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2002-7-10
pubmed:abstractText
alpha-lactorphin (Tyr-Gly-Leu-Phe) lowers blood pressure in conscious adult SHR. This tetrapeptide is originally released from milk protein alpha-lactalbumin by enzymatic hydrolysis. In order to evaluate the antihypertensive mechanisms of alpha-lactorphin, the effects of the tetrapeptide on vascular function were investigated in (30-35 weeks old) spontaneously hypertensive rats (SHR) with established hypertension and age-matched normotensive Wistar-Kyoto (WKY) rats in vitro. In addition, we studied the vascular effects of another structurally related tetrapeptide, beta-lactorphin (Tyr-Leu-Leu-Phe), which originates from milk protein beta-lactoglobulin. Endothelium-dependent relaxation to acetylcholine (ACh) was reduced in mesenteric arterial preparations of SHR as compared to those of WKY. In SHR, the ACh-induced relaxation was augmented by alpha-lactorphin or beta-lactorphin. The role of nitric oxide (NO) is suggested, since this improvement was abolished by the NO synthase (NOS) inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME). Simultaneous potassium channel inhibitor tetraethylammonium (TEA) elicited no additional effect on the ACh-induced relaxation. The cyclooxygenase inhibitor diclofenac did not attenuate the augmented ACh relaxation induced by alpha-lactorphin or beta-lactorphin, suggesting that endothelial vasodilatory prostanoids were not involved in the effect of the tetrapeptides. Endothelium-independent relaxation to the NO donor sodium nitroprusside (SNP) was augmented in mesenteric arterial preparations of SHR by simultaneous beta-lactorphin. The tetrapeptides did not alter vascular responses in mesenteric arteries from WKY. In conclusion, both alpha-lactorphin and beta-lactorphin improved vascular relaxation in adult SHR in vitro. The beneficial effect of alpha-lactorphin was directed towards endothelial function, whereas beta-lactorphin also enhanced endothelium-independent relaxation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0024-3205
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1245-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12106590-Acetylcholine, pubmed-meshheading:12106590-Animals, pubmed-meshheading:12106590-Antihypertensive Agents, pubmed-meshheading:12106590-Blood Pressure, pubmed-meshheading:12106590-Enzyme Inhibitors, pubmed-meshheading:12106590-Hypertension, pubmed-meshheading:12106590-Male, pubmed-meshheading:12106590-Mesenteric Arteries, pubmed-meshheading:12106590-NG-Nitroarginine Methyl Ester, pubmed-meshheading:12106590-Nitric Oxide Donors, pubmed-meshheading:12106590-Nitric Oxide Synthase, pubmed-meshheading:12106590-Nitroprusside, pubmed-meshheading:12106590-Oligopeptides, pubmed-meshheading:12106590-Rats, pubmed-meshheading:12106590-Rats, Inbred SHR, pubmed-meshheading:12106590-Rats, Inbred WKY, pubmed-meshheading:12106590-Vasodilation, pubmed-meshheading:12106590-Vasodilator Agents
pubmed:year
2002
pubmed:articleTitle
Alpha-lactorphin and beta-lactorphin improve arterial function in spontaneously hypertensive rats.
pubmed:affiliation
Institute of Biomedicine, Pharmacology, BIOMEDICUM HELSINKI, P.O. Box 63, FIN-00014 University of Helsinki, Finland. marika.sipola@helsinki.fi
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't