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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
2002-7-24
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pubmed:abstractText |
A hairpin RNA aptamer has been identified by in vitro selection against the transactivation-responsive element (TAR) of HIV-1. A nuclease-resistant N3' --> P5' phosphoramidate isosequential analog of this aptamer also folds as a hairpin and forms with TAR a loop-loop "kissing" complex with a binding constant in the low nanomolar range as demonstrated by electrophoretic mobility-shift assays and surface plasmon resonance experiments. The key structural determinants, which contribute to the stability of the RNA aptamer-TAR complex, loop complementarity and the GA residues closing the aptamer loop, remain crucial for the N3' --> P5' aptamer-TAR complex. Moreover, the N3' --> P5' phosphoramidate aptamer specifically interferes with the binding of a peptide derived from the transactivator protein (Tat) peptide to TAR and selectively inhibits the Tat-mediated transcription in an in vitro assay, which marks this nuclease-resistant aptamer as a relevant candidate for experiments in cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10209261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10212256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10328810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10393900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10496223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10506122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10550206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10562489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10606271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10637358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10801857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10943568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-10995220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-11135542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-11550792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-11563062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-11724578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-2205002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-2470647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-2825027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-3386755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-7539081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-7563092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-8450553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-8637904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-8760881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-9108013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-9193000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-9491887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-9724520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12105271-9739090
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9709-14
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:12105271-Amides,
pubmed-meshheading:12105271-Base Sequence,
pubmed-meshheading:12105271-Gene Products, tat,
pubmed-meshheading:12105271-HIV Long Terminal Repeat,
pubmed-meshheading:12105271-HIV-1,
pubmed-meshheading:12105271-Humans,
pubmed-meshheading:12105271-Kinetics,
pubmed-meshheading:12105271-Molecular Sequence Data,
pubmed-meshheading:12105271-Nucleic Acid Conformation,
pubmed-meshheading:12105271-Phosphoric Acids,
pubmed-meshheading:12105271-Surface Plasmon Resonance,
pubmed-meshheading:12105271-Transcription, Genetic,
pubmed-meshheading:12105271-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2002
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pubmed:articleTitle |
Loop-loop interaction of HIV-1 TAR RNA with N3'-->P5' deoxyphosphoramidate aptamers inhibits in vitro Tat-mediated transcription.
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pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale U386, Université Victor Segalen, 33076 Bordeaux Cédex, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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