Linked Life Data

12105216

Source:http://linkedlifedata.com/resource/pubmed/id/12105216

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
36
pubmed:dateCreated
2002-9-2
pubmed:abstractText
JunD, a member of the Jun family of nuclear transcription proteins, dimerizes with Fos family members or other Jun proteins (c-Jun or JunB) to form the activator protein 1 (AP-1) transcription factor. The junD gene contains no introns and generates a single mRNA. Here we show that two predominant JunD isoforms are generated by alternative initiation of translation, a 39-kDa full-length JunD protein (JunD-FL) by initiation at the first AUG codon downstream of the mRNA 5' cap and a shorter, 34-kDa JunD protein (DeltaJunD) by initiation at a second in-frame AUG codon. The JunD mRNA contains a long, G/C-rich 5'-untranslated region that is predicted to be highly structured and is important for regulating the ratio of JunD-FL and DeltaJunD protein expression. A third functional out-of-frame AUG directs translation from a short open reading frame positioned between the JunD-FL and DeltaJunD start sites. In addition, three non-AUG codons also support translation, an ACG codon (in-frame with JunD) and a CUG are positioned in the 5'-untranslated region, and a CUG codon (also in-frame with JunD) is located downstream of the short open reading frame. Mutation of these start sites individually had no affect on DeltaJunD protein levels, but mutation of multiple upstream start sites led to an increase in DeltaJunD protein levels, indicating that these codons can function cumulatively to suppress DeltaJunD translation. Finally, we show that the JunD mRNA does not possess an internal ribosome entry site and is translated in a cap-dependent manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
32697-705
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12105216-5' Untranslated Regions, pubmed-meshheading:12105216-Animals, pubmed-meshheading:12105216-Base Sequence, pubmed-meshheading:12105216-Binding Sites, pubmed-meshheading:12105216-COS Cells, pubmed-meshheading:12105216-Cell Line, pubmed-meshheading:12105216-Codon, pubmed-meshheading:12105216-Gene Expression Regulation, pubmed-meshheading:12105216-HeLa Cells, pubmed-meshheading:12105216-Humans, pubmed-meshheading:12105216-Luciferases, pubmed-meshheading:12105216-Mice, pubmed-meshheading:12105216-Models, Genetic, pubmed-meshheading:12105216-Molecular Sequence Data, pubmed-meshheading:12105216-Mutagenesis, Site-Directed, pubmed-meshheading:12105216-Mutation, pubmed-meshheading:12105216-Open Reading Frames, pubmed-meshheading:12105216-Phosphorylation, pubmed-meshheading:12105216-Protein Biosynthesis, pubmed-meshheading:12105216-Protein Isoforms, pubmed-meshheading:12105216-Proto-Oncogene Proteins c-jun, pubmed-meshheading:12105216-RNA, Messenger, pubmed-meshheading:12105216-Rats, pubmed-meshheading:12105216-Ribosomes, pubmed-meshheading:12105216-Sequence Homology, Amino Acid, pubmed-meshheading:12105216-Transfection, pubmed-meshheading:12105216-Tumor Cells, Cultured
pubmed:year
2002
pubmed:articleTitle
Translational regulation of the JunD messenger RNA.
pubmed:affiliation
Department of Cell Biology and Biochemistry and the Southwest Cancer Center at University Medical Center, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't