Linked Life Data

12101064

Source:http://linkedlifedata.com/resource/pubmed/id/12101064

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-7-8
pubmed:abstractText
A large amount of oxolinic acid administered in freshwater fish farms reaches the environment. In order to allow environmental monitoring, an HPLC method to determine oxolinic acid in the bryophyte Fontinalis antipyretica was developed. Nalidixic acid was used as an internal standard. Oxolinic and nalidixic acids were extracted by cracking the bryophytes in liquid nitrogen with 0.1 M acid oxalic solution in ethyl acetate and a liquid-liquid clean up procedure was then performed. Mobile phase was a 0.02 M orthophosphoric acid aqueous solution-acetonitrile mixture (70:30, v/v). The stationary phase was 5 microm PuroSpher RP18e and quinolones were detected by fluorescence. The linearity, accuracy and precision of this method were demonstrated by a validation assay. The limits of detection and quantitation were 1 and 10 ng/g respectively. The linearity range was 10 to 500 ng/g. This method was applied to a 25 days experimental study performed with the bryophyte Fontinalis antipyretica.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1570-0232
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Elsevier Science BV.
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
775
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
89-95
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Determination of oxolinic acid in the bryophyte Fontinalis antipyretica by liquid chromatography with fluorescence detection.
pubmed:affiliation
Ecole Nationale Vétérinaire de Nantes, UMR Chimiothérapie Aquacole et Environnement, B.P. 40706, 44307 Nantes, Cedex 03, France. rdelepee@vet-nantes.fr
pubmed:publicationType
Journal Article