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rdf:type |
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lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0019168,
umls-concept:C0023828,
umls-concept:C0032136,
umls-concept:C0301872,
umls-concept:C0332161,
umls-concept:C0679058,
umls-concept:C1442335,
umls-concept:C1522408,
umls-concept:C1547699,
umls-concept:C1704241,
umls-concept:C2700640
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pubmed:issue |
3
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pubmed:dateCreated |
2002-7-8
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pubmed:abstractText |
We hypothesized that the addition of polymer to the surface of liposome/DNA complexes may potentially enhance in vivo delivery of plasmid DNA to antigen-presenting cells and thereby facilitate enhanced immune responses to encoded protein. BALB/c mice were immunized subcutaneously or intramuscularly three times with a total of 50 microg of the plasmid pRc/CMV-HBs(S) (ayw subtype) encoding for the hepatitis B surface antigen. We measured transgene-specific total immunoglobulin G (IgG), IgG2a, IgG2b and IgG1 antibody responses as well as splenocyte and T-cell proliferation and cytokine production upon re-stimulation following immunization. Modification of lipid/DNA complexes by the polymer precipitation method used here for the addition of poly(d,l-lactic acid) was found to be consistently and significantly more effective than either unmodified liposomal DNA or naked DNA in eliciting transgene-specific immune responses to plasmid-encoded antigen when administered by the subcutaneous route. In addition, the polymer-modified formulations delivered by this route were more effective than naked DNA delivered by the intramuscular route in inducing antibody responses (n=5, P<0.03). Our observations provide 'proof of principle' for the use of these multicomponent formulations, which offer potential for manipulation and increased transfection efficiency in vivo for the purposes of genetic immunization.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-10361170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-10528170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-10716608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-10946318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-10992502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-11018552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-11018668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-11312029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-1420259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-1448419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-1532913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-1698714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-2786548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-6722105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-7584951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-8160012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-8265577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-8281146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-8494924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-8806655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-8837611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-8876156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-8987084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-9144463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-9187373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-9227794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-9370932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-9498781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-9500615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-9592627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-9652427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-9743526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12100730-9811479
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
106
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
412-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12100730-Animals,
pubmed-meshheading:12100730-Antibodies, Viral,
pubmed-meshheading:12100730-Cell Division,
pubmed-meshheading:12100730-Cells, Cultured,
pubmed-meshheading:12100730-Cytokines,
pubmed-meshheading:12100730-DNA, Viral,
pubmed-meshheading:12100730-Female,
pubmed-meshheading:12100730-Gene Transfer Techniques,
pubmed-meshheading:12100730-Hepatitis B Surface Antigens,
pubmed-meshheading:12100730-Immunization,
pubmed-meshheading:12100730-Immunoglobulin G,
pubmed-meshheading:12100730-Liposomes,
pubmed-meshheading:12100730-Mice,
pubmed-meshheading:12100730-Mice, Inbred BALB C,
pubmed-meshheading:12100730-Polymers,
pubmed-meshheading:12100730-Spleen,
pubmed-meshheading:12100730-T-Lymphocytes,
pubmed-meshheading:12100730-Transgenes
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pubmed:year |
2002
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pubmed:articleTitle |
Liposome/DNA complexes coated with biodegradable PLA improve immune responses to plasmid encoding hepatitis B surface antigen.
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pubmed:affiliation |
Centre for Drug Delivery Research, School of Pharmacy, University of London, UK.
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pubmed:publicationType |
Journal Article
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