Linked Life Data

12100520

Source:http://linkedlifedata.com/resource/pubmed/id/12100520

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
2002-7-8
pubmed:abstractText
The CDKN2 gene encodes two structurally different proteins: a cyclin-dependent kinase inhibitor, p16, which regulates retinoblastoma protein (pRb)-dependent G1 arrest, and a cell cycle inhibitor, p14ARF, which blocks MDM2-induced p53 degradation resulting in an increase in p53 levels that leads to cell cycle arrest. Recent studies have revealed that expression of p16 and p14ARF is influenced markedly by the status of pRb and p53, and p16 overexpression has been demonstrated in cervical neoplasia because of functional inactivation of pRb by the human papillomavirus (HPV) E7 protein. To clarify the p14ARF status and the relationship between p16/p14ARF and other cell cycle molecules in cervical carcinogenesis, immunohistochemical analysis of p16, p14ARF, p53 and MDM2 was performed on 65 samples of cervical and genital condylomatous and neoplastic lesions, including nine HPV-negative tumors. In most cervical cancers and preneoplastic lesions with HPV infection of high and intermediate risk, a marked overexpression of p14ARF as well as the p16 protein (i.e. dotted nuclear immunostaining) was observed. All condyloma acuminata except one and low-grade dysplasia with HPV infection of low risk, such as HPV 6, immunohistochemically showed completely negative staining for p14ARF, also seen in non-neoplastic and mesenchymal cells. Our results clearly show that the mode of p14ARF overexpression in cervical neoplastic cells with HPV association differs from that in cancers of other organs without HPV association, and the p14ARF overexpression may be attributable to a negative feedback result in the functional inactivation of the pRb and p53 proteins by HPV oncoproteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1320-5463
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
375-83
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12100520-Carcinoma, Squamous Cell, pubmed-meshheading:12100520-Condylomata Acuminata, pubmed-meshheading:12100520-Cyclin-Dependent Kinase Inhibitor p16, pubmed-meshheading:12100520-DNA, Viral, pubmed-meshheading:12100520-Female, pubmed-meshheading:12100520-Genital Diseases, Female, pubmed-meshheading:12100520-Humans, pubmed-meshheading:12100520-Immunohistochemistry, pubmed-meshheading:12100520-In Situ Hybridization, pubmed-meshheading:12100520-Nuclear Proteins, pubmed-meshheading:12100520-Papillomaviridae, pubmed-meshheading:12100520-Papillomavirus Infections, pubmed-meshheading:12100520-Proto-Oncogene Proteins, pubmed-meshheading:12100520-Proto-Oncogene Proteins c-mdm2, pubmed-meshheading:12100520-Tumor Markers, Biological, pubmed-meshheading:12100520-Tumor Suppressor Protein p14ARF, pubmed-meshheading:12100520-Tumor Virus Infections, pubmed-meshheading:12100520-Uterine Cervical Dysplasia, pubmed-meshheading:12100520-Uterine Cervical Neoplasms
pubmed:articleTitle
Overexpression of p16 and p14ARF is associated with human papillomavirus infection in cervical squamous cell carcinoma and dysplasia.
pubmed:affiliation
Second Department of Pathology, Gunma University School of Medicine, Showa-machi, Maebashi, Japan. sanot@gunma-u.ac.jp
pubmed:publicationType
Journal Article