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pubmed-article:12097343pubmed:abstractTextThe publication of a draft of the human genome and of large collections of transcribed sequences has made it possible to study the complex relationship between the transcriptome and the genome. In the work presented here, we have focused on mapping mRNA 3' ends onto the genome by use of the raw data generated by the expressed sequence tag (EST) sequencing projects. We find that at least half of the human genes encode multiple transcripts whose polyadenylation is driven by multiple signals. The corresponding transcript 3' ends are spread over distances in the kilobase range. This finding has profound implications for our understanding of gene expression regulation and of the diversity of human transcripts, for the design of cDNA microarray probes, and for the interpretation of gene expression profiling experiments.lld:pubmed
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pubmed-article:12097343pubmed:articleTitleLong-range heterogeneity at the 3' ends of human mRNAs.lld:pubmed
pubmed-article:12097343pubmed:affiliationOffice of Information Technology, Ludwig Institute for Cancer Research, Switzerland.lld:pubmed
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