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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2002-7-3
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402808,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402809,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402810,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402811,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402812,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402813,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402814,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402815,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402816,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402817,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402818,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402819,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402820,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402821,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402822,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402823,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402824,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402825,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402826,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402827,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402828,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402829,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402830,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402831,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402832,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402833,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402834,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402835,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402836,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402837,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402838,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402839,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402840,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF402841
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pubmed:abstractText |
Ribosomal protein (RP) genes have become widely used as markers for phylogenetic studies and comparative genomics. However, they have not been available for evolutionary studies in fish although teleosts are the largest group of vertebrates with more than 23,000 species. Using a transcriptomic approach, we have cloned and sequenced 32 40S RP complementary DNAs (cDNAs) from channel catfish (Ictalurus punctatus), making them one of the most complete sets of 40S RP gene sequences from a single organism. Most 40S RPs in channel catfish are highly similar to their orthologues in mammalian species, but S19, S21, and S25 are highly divergent. Only one type of cDNA was found for all RP genes except S26 and S27, for which two cDNAs were found in channel catfish. Alternatively spliced transcripts for the S3 and alternatively polyadenylated transcripts for S19 and S21 were found. The 32 40S RP genes are generally highly expressed and together they account for 5.33-11.42% of expression depending on the tissues. Expression levels of the RP genes were highly variable both within a single tissue among different RP genes and among tissues with regard to a single RP gene. Taken together, these data strongly suggest post-transcriptional regulation of RP gene expression, particularly in consideration of the stoichiometry of their representation in ribosomes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
291
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-86
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12095691-Alternative Splicing,
pubmed-meshheading:12095691-Animals,
pubmed-meshheading:12095691-Base Sequence,
pubmed-meshheading:12095691-Brain,
pubmed-meshheading:12095691-DNA, Complementary,
pubmed-meshheading:12095691-Gene Expression,
pubmed-meshheading:12095691-Ictaluridae,
pubmed-meshheading:12095691-Kidney,
pubmed-meshheading:12095691-Mammals,
pubmed-meshheading:12095691-Microsatellite Repeats,
pubmed-meshheading:12095691-Molecular Sequence Data,
pubmed-meshheading:12095691-Poly A,
pubmed-meshheading:12095691-Protein Biosynthesis,
pubmed-meshheading:12095691-Protein Isoforms,
pubmed-meshheading:12095691-Ribosomal Proteins,
pubmed-meshheading:12095691-Sequence Alignment,
pubmed-meshheading:12095691-Sequence Analysis, DNA,
pubmed-meshheading:12095691-Sequence Homology, Nucleic Acid,
pubmed-meshheading:12095691-Skin,
pubmed-meshheading:12095691-Transcription, Genetic
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pubmed:year |
2002
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pubmed:articleTitle |
Translational machinery of channel catfish: I. A transcriptomic approach to the analysis of 32 40S ribosomal protein genes and their expression.
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pubmed:affiliation |
The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Auburn University, 201 Swingle Hall, Auburn, AL 36849, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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