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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0023516,
umls-concept:C0042172,
umls-concept:C0085536,
umls-concept:C0162339,
umls-concept:C0185117,
umls-concept:C0205214,
umls-concept:C0205245,
umls-concept:C0851285,
umls-concept:C1419115,
umls-concept:C1704241,
umls-concept:C2911684
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pubmed:issue |
Pt 2
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pubmed:dateCreated |
2002-7-3
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pubmed:abstractText |
The leucocyte common antigen-related phosphatase (LAR) has been implicated in receptor tyrosine kinase signalling pathways while also displaying cell-density-dependency and localization to adherens junctions. Whereas physiological substrates for LAR have not been identified unequivocally, beta-catenin associates with LAR and is a substrate in vitro. With the implication that LAR may play a role in regulating E-cadherin-dependent cell-cell communication and contact inhibition, the relationship of LAR with E-cadherin was investigated. LAR expression increased with cell density in the human breast cancer cell line MCF-7 and in Ln 3 cells derived from the 13762NF rat mammary adenocarcinoma. LAR protein levels decreased rapidly when cells were replated at a low density after attaining high expression of LAR at high cell density. COS-7 cells displayed comparable density-dependent regulation of LAR expression when transiently expressing exogenous LAR under the control of a constitutively active promoter, indicating that the regulation of expression is not at the level of gene regulation. Disrupting homophilic E-cadherin complexes by chelating extracellular calcium caused a marked decrease in LAR protein levels. Similarly, blocking E-cadherin interactions with saturating amounts of E-cadherin antibody (HECD-1) also led to a rapid and pronounced loss of cellular LAR. In contrast, mimicking cell-surface E-cadherin engagement by plating cells at low density on to dishes coated with HECD-1 resulted in a 2-fold increase in LAR expression compared with controls. These results suggest that density-dependent regulation of LAR expression is mediated by functional E-cadherin and may play a role in density-dependent contact inhibition by regulating tyrosine phosphorylation in E-cadherin complexes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-10187801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-10226590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-10333730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-10425198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-10502299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-10574326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-10681592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-10825293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-10835420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-1639852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-1703631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-3416359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-6950180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-7542250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-7559782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-7576097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-7642713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-7846766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-7929566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-7937872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-8170467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-8425900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-8608551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-8608588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-8707857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-8806677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-8830779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-9020180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-9233779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-9245518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-9245795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-9497337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-9501065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-9647658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-9771479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12095414-9837958
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
365
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
513-9
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:12095414-Animals,
pubmed-meshheading:12095414-COS Cells,
pubmed-meshheading:12095414-Cadherins,
pubmed-meshheading:12095414-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:12095414-Humans,
pubmed-meshheading:12095414-Kinetics,
pubmed-meshheading:12095414-Nerve Tissue Proteins,
pubmed-meshheading:12095414-Protein Tyrosine Phosphatases,
pubmed-meshheading:12095414-Rats,
pubmed-meshheading:12095414-Receptor-Like Protein Tyrosine Phosphatases, Class 2,
pubmed-meshheading:12095414-Receptors, Cell Surface,
pubmed-meshheading:12095414-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
Expression of the leucocyte common antigen-related (LAR) tyrosine phosphatase is regulated by cell density through functional E-cadherin complexes.
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pubmed:affiliation |
Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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