Source:http://linkedlifedata.com/resource/pubmed/id/12091479
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2002-7-1
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| pubmed:abstractText |
Programmed cell death occurs after ischemic, excitotoxic, and traumatic brain injury (TBI). Recently, a caspase-independent pathway involving intranuclear translocation of mitochondrial apoptosis-inducing factor (AIF) has been reported in vitro; but whether this occurs after acute brain injury was unknown. To address this question adult rats were sacrificed at various times after TBI. Western blot analysis on subcellular protein fractions demonstrated intranuclear localization of AIF in ipsilateral cortex and hippocampus at 2-72 h. Immunocytochemical analysis showed AIF labeling in neuronal nuclei with DNA fragmentation in the ipsilateral cortex and hippocampus. Immunoelectronmicroscopy verified intranuclear localization of AIF in hippocampal neurons after TBI, primarily in regions of euchromatin. Large-scale DNA fragmentation ( approximately 50 kbp), a signature event in AIF-mediated cell death, was detected in ipsilateral cortex and hippocampi by 6 h. Neuron-enriched cultures exposed to peroxynitrite also demonstrated intranuclear AIF and large-scale DNA fragmentation concurrent with impaired mitochondrial respiration and cell death, events that are inhibited by treatment with a peroxynitrite decomposition catalyst. Intranuclear localization of AIF and large-scale DNA fragmentation occurs after TBI and in neurons under conditions of oxidative/nitrosative stress, providing the first evidence of this alternative mechanism by which programmed cell death may proceed in neurons after brain injury.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Inducing Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Flavoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pdcd8 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxynitrous Acid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
0022-3042
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| pubmed:author |
pubmed-author:ChenJunJ,
pubmed-author:ClarkRobert S BRS,
pubmed-author:DraviamRomeshR,
pubmed-author:DuLinaL,
pubmed-author:GrahamSteven HSH,
pubmed-author:GuoFengliF,
pubmed-author:KochanekPatrick MPM,
pubmed-author:NathanielPaula DPD,
pubmed-author:SzabóCsabaC,
pubmed-author:WatkinsSimon CSC,
pubmed-author:ZhangXiaopengX
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| pubmed:issnType |
Print
|
| pubmed:volume |
82
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
181-91
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:12091479-Active Transport, Cell Nucleus,
pubmed-meshheading:12091479-Animals,
pubmed-meshheading:12091479-Apoptosis,
pubmed-meshheading:12091479-Apoptosis Inducing Factor,
pubmed-meshheading:12091479-Blotting, Western,
pubmed-meshheading:12091479-Brain Chemistry,
pubmed-meshheading:12091479-Brain Injuries,
pubmed-meshheading:12091479-Cell Nucleus,
pubmed-meshheading:12091479-Cells, Cultured,
pubmed-meshheading:12091479-Cerebral Cortex,
pubmed-meshheading:12091479-DNA Fragmentation,
pubmed-meshheading:12091479-Disease Models, Animal,
pubmed-meshheading:12091479-Disease Progression,
pubmed-meshheading:12091479-Flavoproteins,
pubmed-meshheading:12091479-Hippocampus,
pubmed-meshheading:12091479-Immunohistochemistry,
pubmed-meshheading:12091479-Male,
pubmed-meshheading:12091479-Membrane Proteins,
pubmed-meshheading:12091479-Microscopy, Immunoelectron,
pubmed-meshheading:12091479-Neurons,
pubmed-meshheading:12091479-Peroxynitrous Acid,
pubmed-meshheading:12091479-Rats,
pubmed-meshheading:12091479-Rats, Sprague-Dawley,
pubmed-meshheading:12091479-Subcellular Fractions
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| pubmed:year |
2002
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| pubmed:articleTitle |
Intranuclear localization of apoptosis-inducing factor (AIF) and large scale DNA fragmentation after traumatic brain injury in rats and in neuronal cultures exposed to peroxynitrite.
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| pubmed:affiliation |
Department of Anesthesiology and Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh, 3434 Fifth Avenue, Pittsburgh, PA 15260, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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