Source:http://linkedlifedata.com/resource/pubmed/id/12091405
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2002-7-1
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pubmed:abstractText |
A previous report has described an ocular surface reconstruction method involving the use of cultivated corneal epithelium derived from limbal explants. In the current study, a new culture system was developed involving the in vitro propagation on amniotic membrane (AM) of epithelial cells from enzymatically dissociated limbal epithelium. The purpose of this new method is to produce a cultivated epithelial cell layer that contains stem cells and that is superior to explanted cultivated epithelium. The new cell-suspension technique was compared with the existing explant method.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0146-0404
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2114-21
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12091405-3T3 Cells,
pubmed-meshheading:12091405-Amnion,
pubmed-meshheading:12091405-Animals,
pubmed-meshheading:12091405-Cell Culture Techniques,
pubmed-meshheading:12091405-Cells, Cultured,
pubmed-meshheading:12091405-Epithelial Cells,
pubmed-meshheading:12091405-Epithelium, Corneal,
pubmed-meshheading:12091405-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:12091405-Humans,
pubmed-meshheading:12091405-Keratins,
pubmed-meshheading:12091405-Limbus Corneae,
pubmed-meshheading:12091405-Mice,
pubmed-meshheading:12091405-Microscopy, Electron, Scanning,
pubmed-meshheading:12091405-Microscopy, Fluorescence
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pubmed:year |
2002
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pubmed:articleTitle |
An evaluation of cultivated corneal limbal epithelial cells, using cell-suspension culture.
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pubmed:affiliation |
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kawaramachi-hirokoji, Kamigyo-ku, Kyoto 602-0811, Japan. nkoizumi@ophth.kpu-m.ac.jp
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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