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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5577
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pubmed:dateCreated |
2002-6-28
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pubmed:abstractText |
Phototaxis and photophobic responses of green algae are mediated by rhodopsins with microbial-type chromophores. We report a complementary DNA sequence in the green alga Chlamydomonas reinhardtii that encodes a microbial opsin-related protein, which we term Channelopsin-1. The hydrophobic core region of the protein shows homology to the light-activated proton pump bacteriorhodopsin. Expression of Channelopsin-1, or only the hydrophobic core, in Xenopus laevis oocytes in the presence of all-trans retinal produces a light-gated conductance that shows characteristics of a channel selectively permeable for protons. We suggest that Channelrhodopsins are involved in phototaxis of green algae.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
28
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pubmed:volume |
296
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2395-8
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:12089443-Amino Acid Sequence,
pubmed-meshheading:12089443-Animals,
pubmed-meshheading:12089443-Bacteriorhodopsins,
pubmed-meshheading:12089443-Butyric Acid,
pubmed-meshheading:12089443-Chlamydomonas reinhardtii,
pubmed-meshheading:12089443-Electric Conductivity,
pubmed-meshheading:12089443-Hydrogen-Ion Concentration,
pubmed-meshheading:12089443-Ion Channel Gating,
pubmed-meshheading:12089443-Ion Channels,
pubmed-meshheading:12089443-Ion Transport,
pubmed-meshheading:12089443-Light,
pubmed-meshheading:12089443-Membrane Potentials,
pubmed-meshheading:12089443-Molecular Sequence Data,
pubmed-meshheading:12089443-Oocytes,
pubmed-meshheading:12089443-Patch-Clamp Techniques,
pubmed-meshheading:12089443-Protons,
pubmed-meshheading:12089443-RNA, Complementary,
pubmed-meshheading:12089443-Recombinant Proteins,
pubmed-meshheading:12089443-Retinaldehyde,
pubmed-meshheading:12089443-Sequence Alignment,
pubmed-meshheading:12089443-Temperature,
pubmed-meshheading:12089443-Xenopus laevis
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pubmed:year |
2002
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pubmed:articleTitle |
Channelrhodopsin-1: a light-gated proton channel in green algae.
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pubmed:affiliation |
Max-Planck-Institut für Biophysik, Kennedyallee 70, 60596 Frankfurt am Main, Germany. nagel@mpibp-frankfurt.mpg.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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