12089005

Source:http://linkedlifedata.com/resource/pubmed/id/12089005

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0014833, umls-concept:C0033268, umls-concept:C0049549, umls-concept:C0184511, umls-concept:C0311400, umls-concept:C0679199, umls-concept:C1522240
pubmed:issue	7
pubmed:dateCreated	2002-6-28
pubmed:abstractText	Recently, the feasibility of using Escherichia coli for the heterologous biosynthesis of complex polyketides has been demonstrated. In this report, the development of a robust high-cell-density fed-batch procedure for the efficient production of complex polyketides is described. The effects of various physiological conditions on the productivity and titers of 6-deoxyerythronolide B (6dEB; the macrocyclic core of the antibiotic erythromycin) in recombinant cultures of E. coli were studied in shake flask cultures. The resulting data were used as a foundation to develop a high-cell-density fermentation procedure by building upon procedures reported earlier for recombinant protein production in E. coli. The fermentation strategy employed consistently produced approximately 100 mg of 6dEB per liter, whereas shake flask conditions generated between 1 and 10 mg per liter. The utility of an accessory thioesterase (TEII from Saccharopolyspora erythraea) for enhancing the productivity of 6dEB in E. coli was also demonstrated (increasing the final titer of 6dEB to 180 mg per liter). In addition to reinforcing the potential for using E. coli as a heterologous host for wild-type- and engineered-polyketide biosynthesis, the procedures described in this study may be useful for the production of secondary metabolites that are difficult to access by other routes.
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-10021418, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-10051557, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-10322123, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-10421766, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-11230695, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-11238987, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-11251294, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-11485419, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-1556069, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-2185216, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-9484229, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-9694676, http://linkedlifedata.com/resource/pubmed/commentcorrection/12089005-9756477
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7605801
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/6-deoxyerythronolide B, http://linkedlifedata.com/resource/pubmed/chemical/Erythromycin
pubmed:status	MEDLINE
pubmed:month	Jul
pubmed:issn	0099-2240
pubmed:author	pubmed-author:HuZhihaoZ, pubmed-author:KhoslaChaitanC, pubmed-author:PfeiferBlaineB, pubmed-author:VlasovaV VVV
pubmed:issnType	Print
pubmed:volume	68
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	3287-92
pubmed:dateRevised	2009-11-18
pubmed:meshHeading	pubmed-meshheading:12089005-Erythromycin, pubmed-meshheading:12089005-Escherichia coli, pubmed-meshheading:12089005-Fermentation
pubmed:year	2002
pubmed:articleTitle	Process and metabolic strategies for improved production of Escherichia coli-derived 6-deoxyerythronolide B.
pubmed:affiliation	Department of Chemical Engineering, Stanford University, Stanford, CA 94305-5025, USA.
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't