Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-6-27
pubmed:abstractText
A collection of rhizobia isolated from Acacia tortilis subsp. raddiana from various sites in the North and South of Sahara was analyzed for their diversity at both taxonomic and symbiotic levels. On the basis of whole cell protein (SDS-PAGE) and 16S rDNA sequence analysis, most of the strains were found to belong to the Sinorhizobium and Mesorhizobium genera where they may represent several different genospecies. Despite their chromosomal diversity, most A. tortilis Mesorhizobium and Sinorhizobium symbionts exhibited very similar symbiotic characters. Nodulation tests showed that the strains belong to the Acacia-Leucaena-Prosopis nodulation group, although mainly forming non-fixing nodules on species other than A. tortilis. Most of the strains tested responded similarly to flavonoid nod gene inducers, as estimated by using heterologous nodA-lacZ fusions. Thin layer chromatography analysis of the Nod factors synthesized by overproducing strains showed that most of the strains exhibited similar profiles. The structures of Nod factors produced by four different Sinorhizobium sp. strains were determined and found to be similar to other Acacia-Prosopis-Leucaena nodulating rhizobia of the Sinorhizobium-Mesorhizobium-Rhizobium branch. They are chitopentamers, N-methylated and N-acylated by common fatty acids at the terminal non reducing sugar. The molecules can also be 6-O sulfated at the reducing end and carbamoylated at the non reducing end. The phylogenetic analysis of available NodA sequences, including new sequences from A. tortilis strains, confirmed the clustering of the NodA sequences of members of the Acacia-Prosopis-Leucaena nodulation group.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0723-2020
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
130-45
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12086180-Acacia, pubmed-meshheading:12086180-Acyltransferases, pubmed-meshheading:12086180-Africa, pubmed-meshheading:12086180-Alphaproteobacteria, pubmed-meshheading:12086180-Bacterial Proteins, pubmed-meshheading:12086180-Carbohydrate Sequence, pubmed-meshheading:12086180-Chromatography, Thin Layer, pubmed-meshheading:12086180-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:12086180-Flavonoids, pubmed-meshheading:12086180-Genetic Variation, pubmed-meshheading:12086180-Lipopolysaccharides, pubmed-meshheading:12086180-Models, Molecular, pubmed-meshheading:12086180-Molecular Sequence Data, pubmed-meshheading:12086180-Phylogeny, pubmed-meshheading:12086180-Plant Roots, pubmed-meshheading:12086180-Sequence Analysis, DNA, pubmed-meshheading:12086180-Soil Microbiology, pubmed-meshheading:12086180-Species Specificity, pubmed-meshheading:12086180-Symbiosis
pubmed:year
2002
pubmed:articleTitle
Symbiotic and taxonomic diversity of rhizobia isolated from Acacia tortilis subsp. raddiana in Africa.
pubmed:affiliation
Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD-INRA-CIRAD-ENSAM, Baillarguet, Montpellier, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't