Linked Life Data

12083740

Source:http://linkedlifedata.com/resource/pubmed/id/12083740

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-6-26
pubmed:abstractText
We investigated the effect of black currant (BC) concentrate on smooth muscle in rat thoracic aorta. BC concentrate dose-dependently relaxed the norepinephrine (0.1 microM)-precontracted aorta, and the response was abolished after endothelium removal. Both oxyhemoglobin (1 microM), a nitric oxide (NO) scavenger, and IH-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ, 0.5 microM), an inhibitor of guanylyl cyclase (GC), inhibited the relaxing effect of BC concentrate. NG-nitro-L-arginine methyl ester (L-NAME, 10 microM), a nitric oxide synthase (NOS) inhibitor, inhibited the relaxation, and the subsequent addition of L-arginine (1 mM), a NOS substrate, reversed the inhibitory effects of L-NAME. Neither indomethacin (10 microM), an inhibitor of cyclooxygenase, nor atropine (1 microM), an antagonist of muscarinic receptors, modified the effect of BC concentrate. Diphenhydramine (3 microM) and chlorpheniramine (2 microM), selective antagonists of H1-receptors, inhibited the relaxation, but cimetidine (0.3 mM), a selective antagonist of H2-receptors, did not affect the relaxation. These results indicate that, in the rat aorta, BC concentrate enhances synthesis of NO, which subsequently induces the endothelium-dependent vasorelaxation via the H1-receptors on the endothelium.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-5198
pubmed:author
pubmed:issnType
Print
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-35
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Endothelium-dependent vasorelaxation induced by black currant concentrate in rat thoracic aorta.
pubmed:affiliation
Health & Bioscience Laboratories, Meiji Seika Kaisha, Ltd, Sakado-shi, Saitama, Japan. yuko_nakamura@meiji.co.jp
pubmed:publicationType
Journal Article, In Vitro