Source:http://linkedlifedata.com/resource/pubmed/id/12082260
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2002-6-25
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pubmed:abstractText |
The objective of our research was to reveal the effects of different shear stresses on the expression of proto-oncogenes c-fos and c-myc in cultured human umbilical vein endothelial cells (HUVEC). A parallel plate flow chamber was used to control the value and duration of shear stress (SS), and the expression of c-fos and c-myc protein was measured by immunocytochemistry methods and image analysis software. Some important conclusions were drawn. In the stationary state, c-fos protein levels were very low. The SS's of 4 dyn/cm(2) and 10 dyn/cm(2) induced rapid increases of c-fos protein levels, especially the SS of 10 dyn/cm(2). The levels peaked at 1.0 h. Then, c-fos protein levels began to decrease, after 2.5 h, they declined to almost basal levels. In the stationary state, the c-myc protein levels were also very low slowly increasing after the onset of shear stress. The effects of 4 dyn/cm(2) and 10 dyn/cm(2) SS on c-myc protein expression levels had no difference and apparently were less than the effect on c-fos protein levels. The c-myc protein levels peaked at 1.5 h. Subsequently, they fell to basal levels at 2.5 h. Increased expression of these proto-oncogenes mediated by shear stress may have important effects on the regulation of critical cell activities, such as proliferation and differentiation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1386-0291
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
117-23
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12082260-Blood Circulation,
pubmed-meshheading:12082260-Cells, Cultured,
pubmed-meshheading:12082260-Endothelium, Vascular,
pubmed-meshheading:12082260-Humans,
pubmed-meshheading:12082260-Image Processing, Computer-Assisted,
pubmed-meshheading:12082260-Immunohistochemistry,
pubmed-meshheading:12082260-Kinetics,
pubmed-meshheading:12082260-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:12082260-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:12082260-Stress, Mechanical,
pubmed-meshheading:12082260-Umbilical Veins
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pubmed:year |
2002
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pubmed:articleTitle |
Effects of fluid shear stress on expression of proto-oncogenes c-fos and c-myc in cultured human umbilical vein endothelial cells.
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pubmed:affiliation |
Biomechanics & Medical Information Institute, Beijing Polytechnic University, Beijing, 100022, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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