Linked Life Data

12082252

Source:http://linkedlifedata.com/resource/pubmed/id/12082252

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-6-25
pubmed:abstractText
In this study we investigated the patterns of fluid shear stress on induction of monocyte chemoattractant protein-1 (MCP-1) secretion in cultured human umbilical vein endothelial cells (HUVECs). MCP-1 is a potent special chemoattractant, which recruits monocytes into the sub-endothelium. This process is one of the early events of atherosclerosis. We examined the pattern of fluid shear stress inducing the secretion of MCP-1 in cultured HUVECs from the view of biomechanics. In our experiments, HUVECs were subjected to controlled levels of shear stress (4, 10, 20 dyn/cm(2)) in a parallel plate flow chamber. MCP-1 in HUVECs of different periods was measured by an immunohistochemistry method and digital image analysis; MCP-1 in perfusion was measured by sandwich ELISA. The results demonstrated the increase of MCP-1 synthesis and secretion by shear stress was time- and force-dependent. The accumulated level of MCP-1 in HUVECs under lower shear stress (4 dyn/cm(2)) for 4-5 hrs was 3-fold compared with that for static cells. When the shear stress lasted to 6 hrs, the secretion of MCP-1 was reduced to normal levels and could not be increased even when the shear stress lasted for 12 hours. 10 dyn/cm(2) had less effect on the secretion of MCP-1 compared with 4 dyn/cm(2). This research provides data for understanding the mechanism of the contribution of hemodynamic forces to atherosclerosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1386-0291
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
199-207
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Fluid shear stress induces the secretion of monocyte chemoattractant protein-1 in cultured human umbilical vein endothelial cells.
pubmed:affiliation
Biomedical Engineering Center, Beijing Polytechnic University, Beijing, 100022 China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't